Canrenone

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Oct - Nov 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

Deviations:

no

Principles of method if other than guideline:

not relevant

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Canrenone

EC Number:

213-554-5

EC Name:

Canrenone

Cas Number:

976-71-6

Molecular formula:

C22H28O3

IUPAC Name:

10,13-dimethyl-1,8,9,10,11,12,13,14,15,16-decahydro-3'H-spiro[cyclopenta[a]phenanthrene-17,2'-furan]-3,5'(2H,4'H)-dione

Details on test material:

- Name of test material (as cited in study report): Canrenone
- Analytical purity: 98.4%
- Lot/batch No.: 90515001

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

10 mg/L

Based on:

DOC

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

11

Sampling time:

28 d

Details on results:

The test compound canrenone was degraded to 11 % on day 29 (~ 28 days of incubation). Only
towards the end of the incubation period a slight increase in degradation was observed.

Any other information on results incl. tables

Table 1: Biological degradation (cumulative) in percent (corrected for blank C02 production) of canrenone

Test compound	Nominal concentration	Day of sampling
	of carbon	4	6	8	12	15	20	25	29
Canrenone	10 mg/L	1	1	1	1	1	1	3	11
Reference	10 mg/L	39	63	71	79	85	88	90	93
(sodium acetate)
T oxicity control	10 mg/L +	18	31	36	41	44	46	51	56
(canrenone +
sodium acetate)	10 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

In accordance with the OECD guideline, the test compound canrenone is not readily
biodegradable under the conditions of the test and it was not toxic to the microbes of activated
sludge.

Executive summary:

The test substance canrenone was incubated in an aqueous solution including nutrients with microorganisms from a municipal sewage treatment plant for 28 days (start of treatment = day 1). The nutrient solutions were made up of phosphates, magnesium sulphate, iron chloride, ammonium chloride and calcium chloride. The test substance canrenone was incubated at a concentration of 10 mg carbon/L in triplicate. Additionally, a reference substance (sodium acetate) was tested in a single set according to the same procedure, in order to verify the viability and activity of the degrading microorganisms. One further set was incubated with sodium acetate at 10 mg carbon/L (reference substance) plus canrenone at 10 mg carbon/L representing a toxicity control. Furthermore, a blank control was tested in triplicate without any test or reference substance. The biological degradation of the test and reference substances was evaluated by measurement of the carbon dioxide (C02) produced during the test period. CO2 production was determined on days 4, 6, 8, 12, 15, 20, 25 and 29. On day 28 the solutions were acidified in order to expel all dissolved CO2, and CO2 was determined on day 29. The CO2 production was calculated as the percentage of total CO2 that the test material could theoretically have produced, based on carbon content. The blank CO2 production was subtracted for correction.

The test compound canrenone was degraded to 11 % on day 29 (~ 28 days of incubation). Only towards the end of the incubation period a slight increase in degradation was observed. The reference compound sodium acetate was degraded to 93% on day 29 (~ 28 days of incubation). This demonstrated the viability of the microorganisms.

In the toxicity control, the reference compound (sodium acetate) plus the test compound canrenone, was degraded to 56% on day 29 (~ 28 days of incubation). This reflected the degradation in the individual sets.

In accordance with the OECD guideline, the test compound canrenone is not readily biodegradable under the conditions of the test and it was not toxic to the microbes of activated sludge.