Amines, N-(C16-18 and C18-unsatd. alkyl)trimethylenedi-, diacetates

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

23-05-1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, No certificate of Analyses included.

Justification for type of information:

The category and read-across approach are justified in both documents, please find this further information in the attached documentation part in the dedicated ESR for microorganisms toxicity "Supporting Read-Across document".

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

A stock suspension was used of 1 g/L.
The test was performed using the following test concentrations: Control (duplicate), 6.3, 12.5, 25, 50 and 100 mg/L

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Secondary activated sludge was obtained from the WWTP Nieuwgraaf in Duiven. The WWTP Nieuwgraaf is an actvated sludge plant treating predominantly domestic waste water. The activated sludge concentration in the incubation vessels was 1.0 g DW/L.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Hardness:

Sythetic sewage feed was used: This was made by dissolving 16 peptone, 11 g meat extract, 3 g Urea, 0.7 g NaCl, 0.4 g CaCl2.2H2O, 0.2 g MgSO4.7H2O and 2.8 g K2HPO4 in one liter of deionized water.

Test temperature:

21 °C

pH:

pH ranged from 6.7 to 7.3

Nominal and measured concentrations:

Control (duplicate), 6.3, 12.5, 25, 50 and 100 mg/L nominal

Details on test conditions:

The activated sludge respiration inhibition test was performed according to OECD test guideline 209. In this test the respiration rate of activated sludge fed with a standard amount of synthetic sewage was measured. The respiration rate of the same activated sludge in the presence of various concentrations of the test substance under otherwise identical conditions was also measured.

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

10.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

25.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: 95% conf. lim. 24.6 - 25.7

Duration:

30 min

Dose descriptor:

other: EC80

Effect conc.:

58.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

The control respiration rates are within 0% of each other

Reported statistics and error estimates:

The respiration rate was expressed as a percentage of the mean of two controls of the respiration rate: (1 - 2Rs/(Rc1 + Rc2))*100 = percentage inhibition where: Rs = oxygen consumption rate at tested concentration of the test substance; Rc = oxygen consumption rate of controls.
The EC values were computed from the best fitted lines (least square method) through the points given by the probit of the percentage inhibition and the logarithm of the concentration of the test substance. Confidence limits were computed based on Fieller's Theorem.

Respiration rates of the activated sludge, inhibition percentages, probit values and pH at various concentrations of test substance.

Concentration mg/L	Activity mg O2/min.L	Inhibition %	Probit	pH
Control	0.24	-		6.7
Control	0.24	-		6.7
100.0	0.00	100	8.10	7.2
50.0	0.06	75	5.67	6.8
25.0	0.12	50	5.00	6.8
12.5	0.18	25	4.33	6.8
6.3	0.22	8	4.59	6.8

Validity criteria fulfilled:

yes

Conclusions:

Activated sludge EC50 (30 min) of Duomeen O is EC50 = 25.1 mg/L according to OECD TG 209.

Executive summary:

In order to predict effects of chemicals in the environment and in biological waste water treatment plants, an activated sludge respiration inhibition test was performed. The toxicity to activated sludge was determined in accordance with OECD test guideline 209.

The toxicity of Duomeen O to activated sludge was determined by measuring the respiration ratee after a contact time of 30 minutes at various concentrations of the test substance. The inhibitory effect of Duomeen O at a perticular concentration was expressed as a percentage of the two controls. From the results ECvalues were calcualted. The EC50 of Duomeen O for activated sludge is 25.1 mg/L with 95% CL of 24.6 and 25.7 mg/L. The EC20 and EC80 are 10.7 and 58.7 mg/L resp.

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No GLP, limited details

Justification for type of information:

The category and read-across approach are justified in both documents, please find this further information in the attached documentation part in the dedicated ESR for microorganisms toxicity "Supporting Read-Across document".

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Qualifier:

according to guideline

Guideline:

other: 88/302/EWG (Teil C) EG amtsblatt L 133 (1988), S 118 - 122

GLP compliance:

no

Vehicle:

no

Details on test solutions:

Due to the low solubility of the test substance in water no stock solution was prepared. The definitive test concentrations were directly prepared in the test vessel by weighing. The test concentration range was 15.6 to 1000 mg/L.
A control without test substance or reference substance was included in the procedure.

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Starting concentration: 1600 mg/L based on dry weight
Originating from: communal STP Frankfurt/M-Sindlingen
Pretreatment according to OECD guideline 209
No adaptation applied

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Hardness:

Test medium according to EG guideline

Test temperature:

20 ± 2 ° C

pH:

7.2 ± 0.2

Salinity:

Test medium according to EG guideline

Nominal and measured concentrations:

Nominal quantification is not required

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

66 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

3 h

Dose descriptor:

other: EC20

Effect conc.:

18.6 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

3 h

Dose descriptor:

other: EC80

Effect conc.:

234.8 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

The control and reference tests results were within the required limits

Validity criteria fulfilled:

yes

Conclusions:

EC50 of activated sludge for (Z)-N-9-octadecenylpropane-1,3-diamine is 66.0 mg/L

Executive summary:

The EC50 (3 h) of activated sludge for (Z)-N-9-octadecenylpropane-1,3-diamine is 66.0 mg/L

Description of key information

The 30min_EC50 of N-9-octa-decenyl-, (Z)- propane-1,3-diamine (CAS 7173 -62 -8) to microorganisms is 25.1 mg/L in an OECD TG 209 test. This data has been used as read-across for Amines, N-(C16-18 and C18-unsatd. alkyl)trimethylenedi-, diacetates (CAS 1313206 -64 -2). This is then converted to the corresponding value of the salt, resulting to an EC50 of about 34.2 mg/L (MW of C18-unsaturated diamine = 316, MW of diamine diacetate = 430). 

Key value for chemical safety assessment

EC50 for microorganisms:

34.2 mg/L

Additional information

The lowest EC50 for microorganisms as observed in an OECD 209 test is 25.1 mg/L for N-9-octa-decenyl-, (Z)- propane-1,3-diamine (CAS 7173 -62 -8).

This value is used as a read-across for the N-C16-18-alkyl-(evennumbered) C18 unsaturated) propane-1,3-diamine (CAS 1313206 -64 -2). The substances have the same hydrophilic group and nearly the same hydrophobic group. The only difference concerns the unsaturation level which is higher for (Z)-N-9-octadecenylpropane-1,3-diamine (CAS 7173 -62 -8).

As confirmed by the physico-chemical properties of diamines acetates and fish toxicity result, the read-across from diamines is justified since the presence of acetates has no effect on the ecotoxicity profile of diamines acetates. Thus these toxicity data obtained for microorganisms can be used as read-across for Amines, N-(C16 -18 and C18 -unsatd. alkyl)trimethylenedi-, diacetates (for more details, see the read-across justification document).