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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
no
Analytical monitoring:
no
Remarks:
Stable under normal condition.
Vehicle:
no
Details on test solutions:
The test substance was soluble in water after the process of sonication. Therefore, the stock solution was prepared by dissolving 1000 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) in sonicator. From this stock solution, required test concentration were prepared for achieving the test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L & 200 mg/L, respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Orange City Aquarium, Sitabuldi, Nagpur, MS (India)
- Length at study initiation (length definition, mean, range and SD): average 2.28 cm
- Weight at study initiation (mean and range, SD): average 0.115 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12 hours darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
37.5 mg of CaCO3
Test temperature:
21°C
pH:
7.3
Nominal and measured concentrations:
6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L & 200 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness

Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 25 - < 50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: LC50 (96 hours) Experimental = >25 mg/L and < 50 mg/L
Details on results:
LC50 (96 hours) Experimental = >25 mg/L and < 50 mg/L

8.1. Mortality and symptoms

NominalConcentration

(mg/l)

Number of fish

Mortality (cumulated) after

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

0 (control)

4

4

-

-

-

-

-

-

-

-

6.25

4

4

-

-

-

-

-

-

-

-

12.5

4

4

-

-

-

-

-

-

-

-

25

4

4

-

-

-

-

-

-

-

-

50

4

4

4

2

-

-

-

-

-

-

100

4

4

4

4

-

-

-

-

-

-

200

4

4

4

4

-

-

-

-

-

-

NominalConcentration

(mg/l)

Number of fish

Symptoms after

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

0 (control)

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

6.25

4

4

M4

M4

M4

M4

M4

M4

M4

M4

12.5

4

4

M4

M4

M4

M4

M4

M4

M4

M4

25

4

4

M4

M4

M4

M4

M4

M4

M4

M4

50

4

4

M4

M2

-

M2

-

M2

-

M2

100

4

4

-

-

-

-

-

-

-

-

200

4

4

-

-

-

-

-

-

-

-

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

 

8.2. pH and oxygen content

NominalConcentration

(mg/l)

Number of fish

pH readings after

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

0 (control)

4

4

6.5

6.6

6.5

6.7

7.2

7.2

7.2

7.1

6.25

4

4

6.6

6.6

6.6

6.6

7.1

7.1

7.1

7.1

12.5

4

4

6.5

6.5

6.9

6.9

7.3

7.3

7.0

7.0

25

4

4

6.3

6.3

6.8

6.8

7.2.

7.2

7.3

7.3

50

4

4

6.2

6.2

6.8

6.8

7.2

7.2

7.2

7.2

100

4

4

6.2

6.3

-

-

-

-

-

-

200

4

4

6.3

6.2

-

-

-

-

-

-

NominalConcentration

(mg/l)

Number of fish

Oxygen content (mg/l) after

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

0 (control)

4

4

7.1

7.1

7.1

7.0

6.9

6.9

6.9

6.8

6.25

4

4

7.1

7.0

7.1

7.0

6.9

6.9

6.8

6.8

12.5

4

4

7.1

7.1

7.2

7.1

6.8

6.8

6.7

6.7

25

4

4

7.1

7.1

7.2

7.1

6.7

6.9

6.7

6.8

50

4

4

7.0

7.1

7.0

7.2

6.9

6.8

6.8

6.7

100

4

4

7.1

7.1

-

-

-

-

-

-

200

4

4

7.1

7.1

-

-

-

-

-

-

8.3. Test temperature

NominalConcentration

(mg/l)

Number of fish

Temperature (°C) after

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

0 (control)

4

4

21

21

21

21

21

21

21

21

6.25

4

4

21

21

21

21

21

21

21

21

12.5

4

4

21

21

21

21

21

21

21

21

25

4

4

21

21

21

21

21

21

21

21

50

4

4

21

21

21

21

21

21

21

21

100

4

4

21

21

-

-

-

-

-

-

200

4

4

21

21

-

-

-

-

-

-

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material on Zebra Fish Danio rerio was determine to be >25 mg/L and < 50 mg/L.
Executive summary:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water after the process of sonication. Therefore, the stock solution was prepared by dissolving 1000 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) in sonicator. From this stock solution, required test concentration were prepared for achieving the test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L & 200 mg/L, respectively. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >25 mg/L and < 50 mg/l . Based on the LC50, it can be consider that the chemical was toxicity and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.But as the test substance is rapidly biodegradable , it can be considered to be not classiffied as per CLP criteria.

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water after the process of sonication. Therefore, the stock solution was prepared by dissolving 1000 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) in sonicator. From this stock solution, required test concentration were prepared for achieving the test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L & 200 mg/L, respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be>25 mg/L and < 50mg/l . Based on the LC50, it can be consider that the chemical was toxicity and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.But as the test substance is rapidly biodegradable , it can be considered to be not classiffied as per CLP criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
50 mg/L

Additional information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water after the process of sonication. Therefore, the stock solution was prepared by dissolving 1000 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) in sonicator. From this stock solution, required test concentration were prepared for achieving the test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L & 200 mg/L, respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be>25 mg/L and < 50mg/l . Based on the LC50, it can be consider that the chemical was toxicity and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.But as the test substance is rapidly biodegradable , it can be considered to be not classiffied as per CLP criteria.