Methyl phenylacetate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Analytical monitoring:

no

Remarks:

Stable under normal condition.

Vehicle:

no

Details on test solutions:

The test substance was soluble in water after the process of sonication. Therefore, the stock solution was prepared by dissolving 1000 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) in sonicator. From this stock solution, required test concentration were prepared for achieving the test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L & 200 mg/L, respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Orange City Aquarium, Sitabuldi, Nagpur, MS (India)
- Length at study initiation (length definition, mean, range and SD): average 2.28 cm
- Weight at study initiation (mean and range, SD): average 0.115 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12 hours darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

37.5 mg of CaCO3

Test temperature:

21°C

pH:

7.3

Nominal and measured concentrations:

6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L & 200 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 25 - < 50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: LC50 (96 hours) Experimental = >25 mg/L and < 50 mg/L

Details on results:

LC50 (96 hours) Experimental = >25 mg/L and < 50 mg/L

Sublethal observations / clinical signs:

8.1. Mortality and symptoms

NominalConcentration (mg/l)	Number of fish		Mortality (cumulated) after
			24h			48h		72h		96h
			V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	-		-	-	-	-	-	-	-
6.25	4	4	-		-	-	-	-	-	-	-
12.5	4	4	-		-	-	-	-	-	-	-
25	4	4	-		-	-	-	-	-	-	-
50	4	4	4		2	-	-	-	-	-	-
100	4	4	4		4	-	-	-	-	-	-
200	4	4	4		4	-	-	-	-	-	-

NominalConcentration (mg/l)	Number of fish		Symptoms after
			24h			48h		72h		96h
			V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	NS4		NS4	NS4	NS4	NS4	NS4	NS4	NS4
6.25	4	4	M4		M4	M4	M4	M4	M4	M4	M4
12.5	4	4	M4		M4	M4	M4	M4	M4	M4	M4
25	4	4	M4		M4	M4	M4	M4	M4	M4	M4
50	4	4	M4		M2	-	M2	-	M2	-	M2
100	4	4	-		-	-	-	-	-	-	-
200	4	4	-		-	-	-	-	-	-	-

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

 

8.2. pH and oxygen content

NominalConcentration (mg/l)	Number of fish		pH readings after
			24h			48h		72h		96h
			V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	6.5		6.6	6.5	6.7	7.2	7.2	7.2	7.1
6.25	4	4	6.6		6.6	6.6	6.6	7.1	7.1	7.1	7.1
12.5	4	4	6.5		6.5	6.9	6.9	7.3	7.3	7.0	7.0
25	4	4	6.3		6.3	6.8	6.8	7.2.	7.2	7.3	7.3
50	4	4	6.2		6.2	6.8	6.8	7.2	7.2	7.2	7.2
100	4	4	6.2		6.3	-	-	-	-	-	-
200	4	4	6.3		6.2	-	-	-	-	-	-

NominalConcentration (mg/l)	Number of fish		Oxygen content (mg/l) after
			24h			48h		72h		96h
			V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	7.1		7.1	7.1	7.0	6.9	6.9	6.9	6.8
6.25	4	4	7.1		7.0	7.1	7.0	6.9	6.9	6.8	6.8
12.5	4	4	7.1		7.1	7.2	7.1	6.8	6.8	6.7	6.7
25	4	4	7.1		7.1	7.2	7.1	6.7	6.9	6.7	6.8
50	4	4	7.0		7.1	7.0	7.2	6.9	6.8	6.8	6.7
100	4	4	7.1		7.1	-	-	-	-	-	-
200	4	4	7.1		7.1	-	-	-	-	-	-

8.3. Test temperature

NominalConcentration (mg/l)	Number of fish		Temperature (°C) after
			24h			48h		72h		96h
			V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	21		21	21	21	21	21	21	21
6.25	4	4	21		21	21	21	21	21	21	21
12.5	4	4	21		21	21	21	21	21	21	21
25	4	4	21		21	21	21	21	21	21	21
50	4	4	21		21	21	21	21	21	21	21
100	4	4	21		21	-	-	-	-	-	-
200	4	4	21		21	-	-	-	-	-	-

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material on Zebra Fish Danio rerio was determine to be >25 mg/L and < 50 mg/L.

Executive summary:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water after the process of sonication. Therefore, the stock solution was prepared by dissolving 1000 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) in sonicator. From this stock solution, required test concentration were prepared for achieving the test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L & 200 mg/L, respectively. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >25 mg/L and < 50 mg/l . Based on the LC50, it can be consider that the chemical was toxicity and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.But as the test substance is rapidly biodegradable , it can be considered to be not classiffied as per CLP criteria.

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water after the process of sonication. Therefore, the stock solution was prepared by dissolving 1000 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) in sonicator. From this stock solution, required test concentration were prepared for achieving the test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L & 200 mg/L, respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be>25 mg/L and < 50mg/l . Based on the LC50, it can be consider that the chemical was toxicity and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.But as the test substance is rapidly biodegradable , it can be considered to be not classiffied as per CLP criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

50 mg/L

Additional information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water after the process of sonication. Therefore, the stock solution was prepared by dissolving 1000 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) in sonicator. From this stock solution, required test concentration were prepared for achieving the test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L & 200 mg/L, respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be>25 mg/L and < 50mg/l . Based on the LC50, it can be consider that the chemical was toxicity and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.But as the test substance is rapidly biodegradable , it can be considered to be not classiffied as per CLP criteria.