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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 02 - 09, 2010
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
The study was performer on the freshwater crustacean Daphnia Magna Straus. A strain of this species was originally supplied by the Italian Health Department (Istituto Superiore di Sanità) in September 2004. Since that time, the clone has been bred in ChemService laboratories in reconstituted water with the same characteristic to the water used in the tests and under temperature and light conditions identical to those of the tests. The daphnia breeding was daily fed with a fixed amount of green algae Pseudokirchneriella subcapitata cultivated in the ChemService Laboratory and with a suspension of the yeast Saccharomyces cerevisiae.
At start of the test, the organisms used in the test were 6-24 hours old and were not first brood progeny.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
140 -250 mg/L as CaCO3
Test temperature:
The ambient temperature during the test period ranged between 20.3 and 22.3°C
pH:
The pH of the control after preparation was 7.44 while the pH of test solution immediately after preparation was 4.52, therefore it was corrected before the beginning of the test period by adding 2 drops of a solution of NaOH to obtain a final pH equal to 6.20.
Dissolved oxygen:
During the test period, the dissolved oxygen concentrations in the test medium and the control were always greater than 6.0 mg/L according to guideline that provided a minimum oxygen concentration of 3.0 mg/L.
The oxygen concentration at the end of the test should be ≥ 3 mg/L in control and in the test vessels.

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
IC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
48 h
Dose descriptor:
IC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
24 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.

Any other information on results incl. tables

The test item caused no adverse effect to Daphnia magna after 48 hours exposure at a nominal product concentration of 100 mg/L under static conditions. The NOEC, LOEC and IC50 values after 24 and 48 h, calculated based on nominal concentrations were as follows:

NOEC 100 mg/L

LOEC > 100 mg/L

IC50 > 100 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test item caused no adverse effect to Daphnia magna after 48 hours exposure at a nominal product concentration of 100 mg/L under static conditions. The NOEC, LOEC and IC50 values after 24 and 48 h, calculated based on nominal concentrations were as follows:
NOEC 100 mg/L
LOEC > 100 mg/L
IC50 > 100 mg/L