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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Structure-Toxicity Relationships for the Fathead Minnow, Pimephales promelas: Narcotic Industrial Chemicals
Author:
Gilman D. Veith and Daniel J. Call and L. T. Brooke
Year:
1983
Bibliographic source:
Can. J. Fish Aquat. Sci., 40, 1983, 743-748

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)

Test material

Constituent 1
Chemical structure
Reference substance name:
α,α,2,6-tetrachlorotoluene
EC Number:
201-332-0
EC Name:
α,α,2,6-tetrachlorotoluene
Cas Number:
81-19-6
Molecular formula:
C7H4Cl4
IUPAC Name:
1,3-dichloro-2-(dichloromethyl)benzene

Sampling and analysis

Details on sampling:
The concentration of the test material was measured daily.

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
Source: Environmental Research Laboratory Duluth.
Age at study initiation: 30 days
Weight, mean: 0.12 g
Feeding during test: no feeding during test

Study design

Test type:
flow-through
Total exposure duration:
96 h
Remarks on exposure duration:
Deaths were recorded aftre 1, 3, 6, 12, 24, 48, 96 hours.

Test conditions

Hardness:
Hardness, mean: 45.5 mg/L CaCO3
Test temperature:
25 +/- 1 °C
pH:
Arithmetic mean: 7.5
Dissolved oxygen:
> 60%

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.97 mg/L

Applicant's summary and conclusion

Conclusions:
From this study, LC50 for 2,6-dichlorobenzal chloride was determined to be 0.97 mg/L.