2-Chloro-5-chloromethylpyridine

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Details on study design: HPLC method:

The HPLC method is based on the correlation between the retention of a substance on a reversed phase column packed with a commercially available cyanopropyl solid phase containing lipophilic and polar moieties and the adsorption coefficient Koc.
The dead time is determined by using a homologous series of n-alkyl methyl ketones. For calibration retention times of selected reference compounds with known adsorption coefficients are measured. In a further step the log K0c of the test item should be derived from its retention time.

Chromatographic Conditions
Except for the detection wavelength the chromatographic conditions were the same for the determination of to, the establishment of the calibration graph, and the measurement of the test item.
- HPLC equipment: Kontron HPLC system with autosampler 465, gradient former 425, pump 422 and column oven 480, UV/VIS detector 430; Software Chromeleon Vers. 6.40, Fa. Dionex
- Column: Material: stainless steel (Bischoff), dimensions: 250 x 4 mm, stationary phase: Zorbax CN, 5 µm, column temperature: 40°C
- Mobile Phase: Methanoi / water 55/45 (v/v) (isocratic elution)
- Fiow rate: 1.0 mL/min
- Injected volume: 20 µL
- Detection wavelength: 275 nm (determination of t0), 254 nm (calibration graph), 218 nm (analysis of the test item)
- Injection amounts: n-Alkyl methyl ketones: 154 -172 µg, Calibration substances: 1 - 49 µg, Test item: 1 µg
The alkyl ketones, calibration substances and the test item, respectively, were injected in duplicate as solutions in methanol.

Type:

Koc

Value:

72

Type:

log Koc

Value:

1.86

Conclusions:

The adsorption coefficient of 2-chloro-5-chloromethylpyridine is Koc = 72 and logKoc = 1.86.

Executive summary:

The adsorption coefficient (Koc) of 2 -chloro-5-chloromethylpyridine was determined according to OECD 121 guideline and EEC-guideline 2001/59/EEC C.19. Therefore, eight calibration substances were injected into a HPLC-system under the same analytical conditions (column temperature 40°C) as the test item. A calibration curve was created by using the measured retention times and the known log Koc-values of the calibration substances for linear regression.

The adsorption coefficient of 2-chloro-5-chloromethylpyridine is Koc = 72 and logKoc = 1.86.

Description of key information

The adsorption coefficient (Koc) of 2 -chloro-5-chloromethylpyridine was determined according to OECD 121 guideline and EEC-guideline 2001/59/EEC C.19. Therefore, eight calibration substances were injected into a HPLC-system under the same analytical conditions (column temperature 40°C) as the test item. A calibration curve was created by using the measured retention times and the known log Koc-values of the calibration substances for linear regression.

The adsorption coefficient of 2-chloro-5-chloromethylpyridine is Koc = 72 and logKoc = 1.86.

Key value for chemical safety assessment

Koc at 20 °C:

72