Diethyl sulphide

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics in vivo

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1997

Materials and methods

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

dimethyl sulphide (CAS # 75-18-3)

IUPAC Name:

dimethyl sulphide (CAS # 75-18-3)

Details on test material:

No data available.

Radiolabelling:

no

Test animals

Species:

mouse

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

No data available

Administration / exposure

Route of administration:

inhalation: vapour

Vehicle:

unchanged (no vehicle)

Details on exposure:

Mice weighting about 20g were exposed to an atmosphere containing gaseous DMS in a desiccator. Five different concentrations of DMS gas were investigated and 5 mice were used for each group. Liquid DMS was placed in the bottom of the desiccator which was immediately covered with a lid. The underside was warmed to 40°C in a water bath to vapourize the DMS and the gas mixed with air from the atmosphere with a stirring bar. As DMS was vapourized, the lid was raised slightly by the expanded atmosphere, some of which over-flowed from the desiccator. After the overflow had ceased the lid was opened slightly, a mouse was inserted into the atmosphere and the lid was closed immediately. A small amount of the atmosphere was sampled to determine the concentration of DMS in the atmosphere by inserting a micro-syringe through the adhesive vinyl tape closing a small outlet at the side of the desiccator. The behaviour of the mouse after exposure to the atmosphere was observed and the time elapsed until changes in gait and respiratory arrest were also noted. After death had occurred. The atmosphere was sampled again to determine the concentration of DMS in the atmosphere.

Duration and frequency of treatment / exposure:

Single exposure until death

Doses / concentrations

Remarks:

Doses / Concentrations:
6.8, 11.6, 23.6, 34.0 and 50.6%

No. of animals per sex per dose / concentration:

5

Control animals:

no

Details on study design:

Blood and issue samples were taken, weighed, sealed in class vials and stored at -20°C until quantification of the DMS content was carried out.

Results and discussion

Toxicokinetic / pharmacokinetic studies

Details on distribution in tissues:

DMS was distributed in all tissues of the mice at levels of 0.05-12.9 mg/g with an average of 2.34 mg/g. At a concentration of 6.8 ± 1.3%, DMS was distributed in every tissue almost equally except for the lungs where the concentration was much lower. At concentrations of 11.6 ± 0.5%, 23.6 ± 3.7% and 34.0 ± 3.1 % distribution patterns were almost the same. At a concentration of 30.6 ± 3.6%, DMS was distributed in lung, blood, heart muscle and brain at similar levels to those at 6.8%.

Metabolite characterisation studies

Metabolites identified:

no

Any other information on results incl. tables

Table 1: Distribution of dimethyl sulphide (DMS) in tissues of mice exposed to an atmosphere containing DMS

Tissue	DMS (%)
	6.8 ± 1.3	11.6 ± 0.5	23.6 ± 3.7	34.0 ± 3.1	50.6 ± 3.6
Lung	0.19 ± 0.21	2.90 ± 1.57	1.66 ± 0.73	2.12 ± 0.44	0.29 ± 0.32
Blood	0.76 ± 0.16	5.34 ± 0.94	2.30 ± 0.38	4.54 ± 1.91	0.96 ± 0.29
Heart Muscle	1.20 ± 0.72	3.96 ± 1.16	4.06 ± 1.36	3.78 ± 0.88	1.42 ± 0.36
Brain	1.16 ± 0.36	9.12 ± 2.77	8.22 ± 3.80	8.00 ± 2.92	0.88 ± 0.32
Liver	1.28 ± 0.74	4.50 ± 1.33	2.10 ± 0.97	2.24 ± 0.50	0.20 ± 0.09
Spleen	0.40 ± 0.18	2.30 ± 0.99	1.20 ± 0.47	1.48 ± 0.50	0.20 ± 0.09
Kidney	1.10 ± 0.34	3.62 ± 0.67	1.60 ± 1.08	1.94 ± 0.55	0.48 ± 0.13
Muscle	0.92 ± 0.55	1.52 ± 0.71	1.06 ± 0.26	2.14 ± 1.05	0.38 ± 0.10

Applicant's summary and conclusion

Executive summary:

Following single acute exposure of mice to atmospheres of dimethyl sulfphide vapors from 6.8% to 50.6% (w/v), dimethyl sulphide was distributed in all tissues almost equally, except for the lungs where the concentration was much lower (Terazawa et al., 1991).