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EC number: 229-861-2 | CAS number: 6790-58-5
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- From 07-01-1992 to 22-01-1992
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- This study was performed according to DIN 38412 part 27 with GLP statement, but don't give sufficient experimental details. Therefore, this study is considered not assignable.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412, part 27
- Deviations:
- not specified
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
Test material
- Reference substance name:
- [3aR-(3aα,5aβ,9aα,9bβ)]-dodecahydro-3a,6,6,9a-tetramethylnaphtho[2,1-b]furan
- EC Number:
- 229-861-2
- EC Name:
- [3aR-(3aα,5aβ,9aα,9bβ)]-dodecahydro-3a,6,6,9a-tetramethylnaphtho[2,1-b]furan
- Cas Number:
- 6790-58-5
- Molecular formula:
- C16H28O
- IUPAC Name:
- (3aR,5aS,9aS,9bR)-3a,6,6,9a-tetramethyl-dodecahydronaphtho[2,1-b]furan
- Test material form:
- not specified
- Details on test material:
- - Substance type: clear
- Storage condition of test material: cool and dark until october 1992
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- Not applicable
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances):
- Method: 10 g of the test item was dissolved with 100 mL of deionised water. The test item was stirring for 24 hours with a magnetic stirrer and then, the solution was filtered.
- Eluate: deionised water
- Differential loading: no data
- Control: yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): not applicable
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- No data
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
- Remarks on exposure duration:
- none
- Post exposure observation period:
- none
Test conditions
- Hardness:
- No data
- Test temperature:
- No data
- pH:
- No data
- Dissolved oxygen:
- No data
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- loading rate of 10 g/L.
- Details on test conditions:
- TEST SYSTEM
no data
TEST MEDIUM / WATER PARAMETERS
no data
OTHER TEST CONDITIONS
no data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The respiration rates (oxygen consumption) were measured.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable (limit test)
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study: no. - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 30 min
- Dose descriptor:
- EC0
- Effect conc.:
- >= 10 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Oxygen consumption for the control (mg/L O2/min) = 2.25; 2.25; 2.2 (mean of 2.23 mg/L O2/min).
Oxygen consumption for the test substance (mg/L O2/min) = 2.7 (0% inhibition)
DOC at the beginning of the test = 13.05 mg/L
DOC at the end of the test = 50.1 mg/L. - Results with reference substance (positive control):
- None
- Reported statistics and error estimates:
- None
Any other information on results incl. tables
No additional information
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Remarks:
- no sufficient information.
- Conclusions:
- The test item had no inhibitory effect on the respiration rate of Pseudomonas putida after the incubation period of 30 min at the test item concentration of 10 g/L. The 30min-EC0 was at least 10 g/L.
- Executive summary:
The inhibitory effect of the test item on the respiration rate of Pseudomonas putida was investigated in a 30 min respiration inhibition test according to the DIN Guideline 38412 part 27 with GLP statement.
A limit test with a nominal test concentration of 10 g/L was performed.
The test item had no inhibitory effect on the respiration rate of Pseudomonas putida after the incubation period of 30 min at the test item concentration of 10 g/L. The 30min-EC0 was at least 10 g/L.
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