Copper chloride

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study (OECD)

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Copper Monochloride CAS No: 7758-89-6

Author:

OECD SIDS

Year:

2006

Bibliographic source:

INCHEM 2009

Report date:

2006

Reference 2

Reference Type:

other: Study cited in OECD SIDS

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Copper chloride

EC Number:

231-842-9

EC Name:

Copper chloride

Cas Number:

7758-89-6

Molecular formula:

Cl Cu

IUPAC Name:

λ¹-copper(1+) chloride

Details on test material:

- Name of test material (as cited in study report): Copper monochloride
- Analytical purity: 97%
- Source: Simga-Aldrich corporation
- Lot/batch No.: Lot No. 17119BO

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
no data

ENVIRONMENTAL CONDITIONS
no data

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

other: The test substance was wetted with a saline solution.

Details on dermal exposure:

TEST SITE
- Area of exposure: dorsal part
- % coverage: approximately 10 % of the total body surface area
- Type of wrap if used: porous gauze dressing covered from a non-irritating tape for 24 hours

REMOVAL OF TEST SUBSTANCE
- Washing (if done): residual test substance was removed using a saline solution
- Time after start of exposure: 24 hours

TEST MATERIAL
- For solids, paste formed: yes

Duration of exposure:

24 h

Doses:

2000 mg/kg bw for males; 2000, 1500, 1000, 500 mg/kg bw for females

No. of animals per sex per dose:

5 (in total: 5 males; 25 females)

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Cages of rats were checked at least once a day for any mortality. Animals were individually observed hourly during the first 4 hours after dosing with special attention, and daily for a total of 14 days. The nature and severity of the clinical signs and time were recorded at each observation.
The body weight of each rat was recorded on day 1 (prior to dosing), day 8 and day 15 (prior to necropsy). Individual body weight changes were calculated.
- Necropsy: All animals were killed on day 15 by carbon dioxide asphyxiation and subject to a gross necropsy. All gross pathological changes were recorded for each animal. Microscopic examination was not conducted because evidence of gross pathlogical changes was not showed.

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

1 224 mg/kg bw

95% CL:

1 032 - 1 453

Mortality:

Males showed no death, but females showed 4 deaths in 2000 mg/kg bw, 4 deaths in 1500 mg/kg bw, and 1 death in 1000 mg/kg bw, respectively.

Clinical signs:

other: It was observed that symptom of the hardness of skin, an exudation of hardness site, formation of scar/falling off scar, and reddish change in application sites. It was considered to relate with application of test substance. A reddish or black urine was

Gross pathology:

In macroscopic examination, on application sites, the died males showed hardness of skin and acute rats showed necrosis and hemorrhage in subcutaneous. At test finished, it was observed crust of applied site in necropsyp rat. The higher dose concentration, this symptom was severely observed. It was found to be appeared inflammatory response of applied site caused by test substance and toxicity by it. Internally, no abnormality was observed in all animals. In 1500 mg/kg bw of 1 female, size of kidney, adrenal gland and spleen were enlarged and lung became smaller, but it was not considered test substance’s effect but lesion showed in rodent.

Any other information on results incl. tables

When given copper monochloride to rats by dermal application, it was found to cause skin inflammation and injury. 
In case of being absorbed by dermal, it was found to cause systemic toxicity symptoms. 
Female rats appeared to be more susceptible to copper monochloride than males based on mortality and clinical signs.

 

Applicant's summary and conclusion

Conclusions:

CL-Freetext: