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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-guideline study, non-GLP, available as unpublished report, limitations in design and/or reporting but otherwise adequate for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report Date:
1978

Materials and methods

Principles of method if other than guideline:
10 Sprague-Dawley rats per sex per dose were exposed to 4.8 or 6.0 mg/L of the test substance for 4 hours by inhalation. After exposure, the animals were observed for 14 days.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2,5-Dihydrofuran
- Substance No.: 77/746
- Physical state: clear liquid
- Purity: 93%

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: Wiga, Sulzfeld
- Weight at study initiation: 190 and 220 g, for females and males respectively
- Diet: ad libitum, Herilan MRH (H.Eggermann KG, Rinteln/Weser)
- Water: tap water ad libitum

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
air
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Rate of air: 3 L/h
- Exposure chamber volume: 180 L

TEST ATMOSPHERE
- Brief description of analytical method used: gas chromatography
- Samples taken from breathing zone: yes
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
4.8 and 6 mg/L air
No. of animals per sex per dose:
10
Control animals:
yes
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight
- Frequency body weight determination: before the study, 7 days after exposure and at the end of the study

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 6 mg/L air (analytical)
Based on:
test mat.
Exp. duration:
4 h
Mortality:
No mortality observed.
Clinical signs:
- 4.8 mg/L: Eyelid closure
- 6.0 mg/L: Eyelid closure and slightly red nose area
Body weight:
There was no difference in the body weight gain between the control group and the experimental groups.
Gross pathology:
No effects observed.

Applicant's summary and conclusion