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EC number: 216-088-0 | CAS number: 1493-27-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well conducted but not under GLP. No negative controls.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- no negative controls
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 1-fluoro-2-nitrobenzene
- EC Number:
- 216-088-0
- EC Name:
- 1-fluoro-2-nitrobenzene
- Cas Number:
- 1493-27-2
- Molecular formula:
- C6H4FNO2
- IUPAC Name:
- 1-fluoro-2-nitrobenzene
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): 2-fluoronitrobenzene
- Physical state: liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Animals were supplied by Bantin and Kingman Ltd., Hull, yorks.
- Age at study initiation: no data
- Weight at study initiation: 160 +/- 20 g
- Fasting period before study: no
- Housing: The animals were housed separately during the 24 hour contact period, and thereafter according to dosage group and sex, in grid-floor cages.
- Diet: Standard pelleted laboratory animal diet (41B from E. Dixon and Son (Ware) Ltd., Herts) was provided ad libitum.
- Water: ad libitum
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/-2
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod: 12 hrs dark / 12 hrs light
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- maize oil
- Details on dermal exposure:
- TEST SITE
- Area of exposure: the back and flanks of each animal.
- Type of wrap if used: Immediately after administration the trunk of the rat was encircled by a strip of impermeable adhesive tape lined with aluminium foil, to occlude the application site.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): After a contact period of 24 hours the occlusive strip was removed and the contaminated area of skin cleansed of residual test material as far as possible by wiping with a soluion of detergent and warm water. The site was then rinsed and dried.
- Time after start of exposure: 24 hours
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.25 ml/100 g body weight
- Constant volume or concentration used: yes - Duration of exposure:
- 24 hours
- Doses:
- Range finding assay: 1, 0.5 and 0.25 ml/kg (corresponding to 1337, 669 and 334 mg/kg bw)
Final assay: 0.70, 0.50, 0.35 and 0.25 ml/kg (corresponding to 936, 669, 468 and 334 mg/kg bw)
To convert the dose values the density (1.3375 g/ml) of the substance has been used: y ml/kg x 1.3375 g/ml = z g/kg bw. - No. of animals per sex per dose:
- Range finding assay: 2 animals per group
Final assay: 5 males and 5 females per group. - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days (main test) 7 days (range finding study)
- Frequency of observations and weighing: The animals were inspected at daily intervals for a period of 14 days after dosing. Any deaths and other signs of toxicity were recorded, and body weight was also monitored throughout the experimental period.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight. - Statistics:
- The acute dermal LD50 value, together with its 95% confidence limits, was calculated according to the method of Litchfield and Wilcoxon - A Simplified Method of Evaluating Dose - Effect Experiments - J. Pharm. Exp. Tharap. 1949, 96 99.
Results and discussion
- Preliminary study:
- A range-finding study was conducted involving the administration of the product at dose levels equivalent to 1, 0.5 and 0.25 ml/kg (corresponding to 1337, 669 and 334 mg/kg) to groups of 2 animals to determine the order of acute dermal toxicity with respect to lethal effect. These animals were observed immediately after dosing and then throughout the following 7 day period. Mortality results are presented in table 1 in the field "any other information on results incl. tables".
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 535 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 428 - 669
- Mortality:
- Yes, mortality occurred during the time course of the test. For full details see table 2 in the field "any other information on results incl. tables".
- Clinical signs:
- other: At 936, 669 and 468 mg/kg bw, ataxia, marked piloerection, salivation, hypotermia, diuresis, chromodacryorrhoea, blood on masks and coma were observed within 24 hours of dosing. In general, animals surviving beyond day 4 of the test were apparently asympt
- Gross pathology:
- The lung of all the animals examined were found to be congested with blood. Certain individuals exibited discolouration of the liver which may possibly have been due to autolysis.
- Other findings:
- None
Any other information on results incl. tables
Table 1: Mortality results of the range finding assay
Dose level (mg/kg bw) | Deaths by day | Percentage Mortality (%) |
||||
1 | 2 | 3 | 4 | 7 | ||
1337 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 100 |
669 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 100 |
334 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0 |
Table 2: Mortality results of the final assay
Dose level (mg/kg bw) | Deaths by day | Percentage Mortality (%) |
||||||
1 | 2 | 3 | 4 | 5 | 7 | 14 | ||
936 | 5/10 | 8/10 | 9/10 | 9/10 | 9/10 | 9/10 | 9/10 | 90 |
669 | 3/10 | 7/10 | 7/10 | 7/10 | 7/10 | 7/10 | 7/10 | 70 |
468 | 2/10 | 4/10 | 4/10 | 4/10 | 4/10 | 4/10 | 4/10 | 40 |
334 | 0/10 | 0/10 | 0/10 | 0/10 | 0/10 | 0/10 | 0/10 | 0 |
Table 3: Group mean body weight data
Dose level (mg/kg bw) | Body weight (g) at day | |||
0 | 3 | 7 | 14 | |
936 | 162.7 | 150.0 | 168.0 | 186.0 |
669 | 158.2 | 170.7 | 197.3 | 231.3 |
468 | 162.5 | 150.5 | 187.3 | 214.7 |
334 | 154.7 | 166.8 | 180.3 | 212.9 |
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- Under the conditions of this test, the results indicated that the test substance should be classified as Acute dermal Tox. 3, H311 (Toxic in contact with skin) according to CLP.
- Executive summary:
In an acute toxicity test, wistar rats of both sexes (5 males and 5 females per group) were exposed to 2 -Fluoronitrobenzene. The sample was administered at dose levels equivalent to 936, 669, 468 and 334 mg/kg bw, in order to obtain the 0 - 100 % sequence of lethal effects necessary for the estimation of the acute dermal LD50 value. Animals were observed immediately following dosing and thereafter at daily interval for a period of 14 days. Any deaths or other signs of toxicity were recorded. Body weight was monitored throughout the study, and postmortem examinations were performed on selected individuals to observe any gross abnormalities in the viscera. At 936, 669 and 468 mg/kg bw, ataxia, marked piloerection, salivation, hypotermia, diuresis, chromodacryorrhoea, blood on masks and coma were observed within 24 hours of dosing. In general, animals surviving beyond day 4 of the test were apparently asymptomatic by that time. At 334 mg/kg bw, piloerection, loss of activity, hypotermia, salivation, blood on mask and chromodacryorrhoa were exhibited 24 hours after administration of the test material. Individuals in the groups at 936 and 468 mg/kg bw exhibited weight loss at day 3 of the test period. However, the majority of these rats showed normal weight gain by day seven of the experiments. The lung of all the animals examined were found to be congested with blood. Certain individuals exibited discolouration of the liver which may possibly have been due to autolysis. Mortality occurred in 90 % of animals at the highest dose (936 mg/kg bw). At 669 mg/kg bw, 70 % of mortality was observed and at the dose level of 468 mg/kg bw 40 % of mortality was observed. At the lowest dose (334 mg/kg bw), no mortality was observed. Then, a LD50 value of 535 mg/kg bw (428 - 669 mg/kg bw) has been calculated using the method of Litchfield and Wilcoxon. Based on results of this acute test, the substance is classified as Acute dermal Tox. 3, H311 (Toxic in contact with skin) according to CLP.
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