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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Description of key information

With high probability acutely not harmful to fish.

Key value for chemical safety assessment

Additional information

A 96-h acute toxicity study was conducted with N,N,N',N'-Tetramethylhexamethylenediamine (TMHDA, CAS 111 -18 -2) in accordance with OECD guideline 203 and EEC Directive 92/69, Part C.1. to determine the ability of the test substance to produce toxic effects and determine the LC50 at all observation timepoints. The test was conducted with carp exposed to concentration ranging from 10 to 100 mg/L in a semi-static test system with daily renewal of the test solutions. The LC50 for carp exposed to TMHDA was 75 mg/L (nominal, analytically verified) with 0% mortality at 56 mg/L and 100% mortality at 100 mg/L (Tosoh Europe B.V., 1995, report no. 146385). These mortality results were observed after 24 hours of exposure. No additional mortality was recorded during the remaining testing period.

In addition, a GLP-guideline study by BASF AG (1987, report no. 10F0181/875068) was performed, testing the acute toxicity of N,N,N',N'-Tetramethylhexamethylenediamine towards Leuciscus idus. The concentrations were not analytically verified, but TMHDA is expected to be stable in water, due to the high water solubility (BASF SE 2013, report no. 11L00324), the moderate vapor pressure (VP = 0.124 hPa @20°C, Notox Project 2006, report no. 461407) and the low Henry's Law Constant (HLC = 7.57 E-03 Pa m³/mol @ 25°C, calculated with HENRYWIN v3.20, not in applicability domain, BASF SE 2016) of the substance.

No effect was observed in pH-adjusted test solution with a concentration of 100 mg/L. Thus, the mortality in the study mentioned above is considered to be caused by pH. Therefore, the LC50 is supposed to be higher than 100 mg/L at environmentally relevant pH values.

In conclusion, TMHDA is considered to be with high probability acutely not harmful to fish.