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EC number: 236-948-9 | CAS number: 13560-89-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Repeated dose toxicity: oral
Administrative data
- Endpoint:
- sub-chronic toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 90 days
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Only purity and physical chemical properties of test substance not given.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 975
- Report date:
- 1975
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 408 (Repeated Dose 90-Day Oral Toxicity Study in Rodents)
- Deviations:
- yes
- Remarks:
- no ophthalmoscopy was performed
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- 1,6,7,8,9,14,15,16,17,17,18,18-dodecachloropentacyclo[12.2.1.16,9.02,13.05,10]octadeca-7,15-diene
- EC Number:
- 236-948-9
- EC Name:
- 1,6,7,8,9,14,15,16,17,17,18,18-dodecachloropentacyclo[12.2.1.16,9.02,13.05,10]octadeca-7,15-diene
- Cas Number:
- 13560-89-9
- Molecular formula:
- C18H12Cl12
- IUPAC Name:
- 1,2,3,4,7,8,9,10,13,13,14,14-dodecachloro-1,4,4a,5,6,6a,7,10,10a,11,12,12a-dodecahydro-1,4:7,10-dimethanodibenzo[a,e][8]annulene
- Test material form:
- solid: particulate/powder
- Details on test material:
- Dechlorane Plus 25, no further details reported
Particle Size Distribution
TI, for weeks 1 through 4
Total number counted: 330, 433, 507, 386 µm
smallest particles observed: 2.5, 2.5, 2.0, 2.5 µm
largest particles observed: 110, 131, 112, 106 µm
TII, for weeks 1 through 4
Total number counted: 362, 411, 433, 421 µm
smallest particles observed: 2.0, 2.5, 2.5, 2.5 µm
largest particles observed: 130, 154, 142, 159 µm
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Charles River
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Rats were included in the test at an age of 28 days. No information on environmental conditions reported.
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- Dechlorane Plus 25 was blended into the diet in a high-speed blending apparaus. Fresh diets were prepared each week. The diet was offered ad libitum.
- Analytical verification of doses or concentrations:
- no
- Duration of treatment / exposure:
- 90 days
- Frequency of treatment:
- daily
Doses / concentrationsopen allclose all
- Dose / conc.:
- 0 ppm
- Remarks:
- Basis: nominal in diet
- Dose / conc.:
- 10 000 ppm
- Remarks:
- Basis: nominal in diet
- Dose / conc.:
- 30 000 ppm
- Remarks:
- Basis: nominal in diet
- Dose / conc.:
- 100 000 ppm
- Remarks:
- Basis: nominal in diet
- No. of animals per sex per dose:
- 15 males and 15 females per dose
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- Groups of 15 male and 15 female rats were given diet containing Dechlorane Plus 25 at 0, 10,000, 30,000, or 100,000 ppm daily for 90 days.
- Positive control:
- none
Examinations
- Observations and examinations performed and frequency:
- mortality and clinical signs of toxicity daily, food consumption weekly, bodyweights weekly, haematology and clinical chemistry including urinalysis on days 45 and 84, macroscopic pathology including organ weight determination and microscopic pathology at the end of treatment.
- Sacrifice and pathology:
- All rats were subjected to necropsy and organ weight determination (weights of brain, gonads, heart, kidneys, liver, and spleen), in control animals and animals of the high dose group, microscopic pathology examination was performed. A total of 37 organs and tissues were examined histologically.
- Statistics:
- Analysis of variance, Tukey's or Scheffe's Multiple Comparison tests, Kruskel-Wallis Statistics and Multiple Comparisons.
Results and discussion
Results of examinations
- Clinical signs:
- no effects observed
- Description (incidence and severity):
- 2 deaths accidental at blood collection in the high dose group
- Mortality:
- no mortality observed
- Description (incidence):
- 2 deaths accidental at blood collection in the high dose group
- Body weight and weight changes:
- no effects observed
- Food consumption and compound intake (if feeding study):
- no effects observed
- Food efficiency:
- no effects observed
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- no effects observed
- Clinical biochemistry findings:
- no effects observed
- Urinalysis findings:
- no effects observed
- Behaviour (functional findings):
- not examined
- Organ weight findings including organ / body weight ratios:
- no effects observed
- Gross pathological findings:
- effects observed, treatment-related
- Description (incidence and severity):
- slightly increased liver weight at the high dose
- Histopathological findings: non-neoplastic:
- no effects observed
- Histopathological findings: neoplastic:
- no effects observed
- Details on results:
- At the high dose level, slightly increased liver weight was seen without histological correlate. No other test substance related effects occurred.
Effect levels
- Key result
- Dose descriptor:
- NOAEL
- Effect level:
- >= 100 000 ppm
- Based on:
- act. ingr.
- Sex:
- male/female
- Basis for effect level:
- other: Besides slightly increased liver weights at the highest dose level without histological correlate, no test substance related effects at all were seen.
Target system / organ toxicity
- Critical effects observed:
- not specified
Applicant's summary and conclusion
- Conclusions:
- Dechlorane Plus 25 was essentially nontoxic upon administration in the diet at concentrations up to 10000 ppm.
- Executive summary:
Dechlorans Plus 25 was administered to groups of 15 male and 15 female rats in the diet at concentrations of 0, 10,000, 30,000, or 100,000 ppm daily for 90 days. The following parameters were recorded: mortality, clinical signs of toxicity, bodyweights, food consumption, haematology, clinical chemistry and urinalysis, macroscopic and microscopic pathology including organ weights. Besides slightly increased liver weight without histological correlate, thus indicating a functional adaption of the liver to an increased metabolic load, no substance related effects were seen. Dechlorane Plus was essentially nontoxic. The NOAEL was determined at 100,000 ppm in diet corresponding to about 5,870 mg/kg bw for males and to about 7,670 mg/kg bw for females.
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