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EC number: 200-522-0 | CAS number: 61-90-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
No test results for L-leucine are available. However, based on test results available for read-across substance L-valine it can be concluded that acute toxicity to fish is not expected to occur upon exposure to up to 1000 mg/L of L-leucine.
Key value for chemical safety assessment
Additional information
No test results for L-leucine are available. However, read-across to the test results available for structural analogue L-valine is deemed justified based on a comparison of the main factors driving environmental toxicity: pH, water solubility, log Kow and chemical reactivity (functional groups).
- both are essential amino acids that are in the zwitterion state at physiological pH
- the chemical structure differs only in that L-leucine has one extra methylene group in the aliphatic side chain
- the pKa values of the α-COOH group is very similar: 2.36 for LEU, 2.32 for VAL
- the pKa values of the α-NH2 group is very similar: 9.60 for LEU, 9.62 for VAL
- the substances both have a high water solubility: 23 g/L for LEU, 58 g/L for VAL
- the substance both have a low log Kow value: -1.59 for LEU (calculated by EpiWin QSAR), -2.08 for VAL (calculated by EpiWin QSAR)
As the chemical structure and reactivity, and the water solubility, log Kow and pKa values of L-leucine and L-valine are almost identical, it can be concluded that read-across for aquatic toxicity information between those 2 substances is acceptable.
The tests available for L-valine are carried out according to OECD guidelines and reveal no fish mortality at the highest tested concentrations (1000 mg/L for the key study, 10000 mg/L for the supporting study. As a consequence, acute toxicity to fish is not expected to occur upon exposure to the L-leucine up to a concentration of 1000 mg/L.
This is further confirmed by the result of the QSAR (EcoSAR, EpiSuite) that predicted an 96h-LC50 of > 33000 mg/L for L-leucine.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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