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Environmental fate & pathways

Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of method:
HPLC estimation method
Media:
soil/sewage sludge
Test temperature:
25 °C (column temperature)
Details on study design: HPLC method:
EQUIPMENT
- Apparatus:
- Type: Agilent 1200 Series Liquid Chromatograph
- Type, material and dimension of analytical (guard) column: HyperClone CN (CPS) (25 cm x 4.6 mm internal diameter)
- Detection system: UV set at 210 nm

MOBILE PHASES
- Type: Acetonitrile:tetrahydrofuran:water (30:25:45 v/v/v)
- Experiments with additives carried out on separate columns: no additives used
- pH: no data
- Solutes for dissolving test and reference substances: HPLC mobile phase

DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column (formamide)

REFERENCE SUBSTANCES
- Identity: Acetanilide, Nitrobenzene, Naphthalene, 2-Methylnaphthalene, Anthracene, DDT
- Known logKoc values: 1.3, 2.4, 2.8, 3.9, 4.4, 5.6

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 0.5 µg
- Quantity of reference substances:
Formamide (internal deadtime standard): 4.1 µg, Acetanilide: 0.2 µg, Nitrobenzene: 0.2 µg, Naphthalene: 0.1 µg, 2-Methylnaphthalene: 0.1 µg, Anthracene: 0.2 µg, DDT: 0.4 µg
- Intervals of calibration: before and after injections of the test substance

REPETITIONS
- Number of determinations: two

EVALUATION
- Determination of the log Koc value: Calibration plots of log10k versus log10Koc were constructed for the reference standards. The logarithm of the capacity factor for the test substance was calculated and the log10Koc value was determined by interpolation.
Key result
Type:
log Koc
Value:
2.4
Temp.:
25 °C
Validity criteria fulfilled:
not applicable

Description of key information

Log Koc at 25 °C: 2.4 (HPLC method; OECD TGD 121, EU Method C.19)

Key value for chemical safety assessment

Koc at 20 °C:
251

Additional information

WS400517 has a Log Koc = 2.4 at 25 °C and thus no high potential for adsorption to sediment, soil, sewage sludge.

[LogKoc: 2.4]