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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
other information
Study period:
from 7-7-1988 to 15-7-1988
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2,3-dichloroaniline
EC Number:
210-157-9
EC Name:
2,3-dichloroaniline
Cas Number:
608-27-5
Molecular formula:
C6H5Cl2N
IUPAC Name:
2,3-dichloroaniline
Details on test material:
- Name of test material (as cited in study report): 2,3-dichloroaniline
- Physical state: solid (dark brown)
- Analytical purity: 99.1%
- Purity test date: 15-4-1988
- Lot/batch No.: 3
- Storage condition of test material: refrigerator

Method

Species / strain
Species / strain / cell type:
other: Salmonella typhimurium TA1535, TA100, TA1537, TA 98
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
S9 mix
Test concentrations with justification for top dose:
0, 20 - 12500 µg/plate dissolved in DMSO
Controlsopen allclose all
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
sodium azide
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
other: 4-nitro-1,2-phenylene-diamine; 2-aminoanthracene; nitrofurantoin

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
at 300 µg/plate and above
Vehicle controls validity:
valid
Untreated negative controls validity:
not examined
Positive controls validity:
valid
Remarks on result:
other: other: Salmonella typhimurium TA1535, TA100, TA1537, TA 98
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion