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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented report which meets basic scientific principles

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976
Report date:
1976

Materials and methods

Principles of method if other than guideline:
15 rats/sex received a singel oral application per gavage of 250, 500, 1000, 1500, 2000, 2500, 2500 3000, 3500 or 5000 mg/kg bw - male rates resp. 500, 1000, 1500, 2000, 2500, 3000, 3250 or 3500 mg/kg bw - female rats, of the test substance. Post-exposure period was 14 days.
GLP compliance:
no
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
2,3-dichloroaniline
EC Number:
210-157-9
EC Name:
2,3-dichloroaniline
Cas Number:
608-27-5
Molecular formula:
C6H5Cl2N
IUPAC Name:
2,3-dichloroaniline
Details on test material:
- Name of test material (as cited in study report): 2,3-dichloroaniline
- Analytical purity: not reported (technically pure)

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
male + female Wister rats, weight 160-180 g, husbandry: standardised conditions, cage (makrolon type III)

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
polyethylene glycol
Details on oral exposure:
2,3-dichloroaniline was emulsified in lutrol (polyethylenglycol 400), animals received 0.5 ml of the emulsion per 100 g bw
Doses:
m: 250, 500, 1000, 1500, 2000, 2500, 2600, 3000, 3500, 5000 mg/kg bw;
f: 500, 1000, 1500, 2000, 2500,3000, 3250, 3500 mg/kg bw
No. of animals per sex per dose:
15
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LD50
Effect level:
2 635 mg/kg bw
Sex:
female
Dose descriptor:
LD50
Effect level:
2 489 mg/kg bw

Any other information on results incl. tables

Clinical signs:
reduced general condition,difficulties in breathing;
rats with higher dosages revealed additionally tremor, cyanotic discoloration of skin and visible mucous membranes:

dosages in mg/kg bw: number of dead rats/number of rats with clinical signs/number of rats used/time of death:
Males: // Females:
0250: 0 / 0 / 15 / --......// ---------------
0500: 1 / 15 / 15 / 5d......// 0 / 0 / 15 / --
1000: 0 / 15 / 15 / --......// 0 / 15 / 15 / --
1500: 0 / 15 / 15 / --......// 1 / 15 / 15 / 2h
2000: 3 / 15 / 15 / 1d......// 6 / 30 / 30 / 1h-2d
2500: 4 / 15 7 15 / 18'-1d..// 5 / 15 / 15 / 2h-3d
2600: 8 / 15 / 15 / 1h-1d...//....---------------
3000: 10 / 15 / 15 / 1d......// 10 / 15 / 15 / 2h-1d
3250: ---------------------..// 14 / 15 / 15 / 1h-1d
3500: 14 / 15 / 15 / 2h-1d...// 15 / 15 / 15 / 3h-2d
5000: 15 / 15 / 15 / 1h-1d...// --------------------

calculation:
LD50 (male): 2635 mg/kg bw
LD50 (female): 2489 mg/kg bw

Applicant's summary and conclusion