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Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

Administrative data

Endpoint:
biodegradation in water: sediment simulation testing
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented publication which meets basic scientific principles

Data source

Reference
Reference Type:
publication
Title:
Reductive Dehalogenation of Chloroanilines in Anaerobic Estuarine Sediment
Author:
Susarla S, Yonezawa Y, Masunaga S
Year:
1997
Bibliographic source:
Environmental Technology 18: 75 - 83

Materials and methods

Principles of method if other than guideline:
other: no guideline followed; see Test Conditions
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2,3-dichloroaniline
EC Number:
210-157-9
EC Name:
2,3-dichloroaniline
Cas Number:
608-27-5
Molecular formula:
C6H5Cl2N
IUPAC Name:
2,3-dichloroaniline
Details on test material:
Name of test material (as cited in publication): 2,3-DCA.
No purity stated.
The test material was purchased from Tokyo Kasei Kogyo Co. Ltd., (TCI), Tokyo, Japan and were used without further purification.
Stock solution: 2x10^-3 moles/L in methanol.
Radiolabelling:
no

Study design

Oxygen conditions:
anaerobic
Inoculum or test system:
natural sediment
Details on source and properties of sediment:
The sediment used in the experiment was collected at the mouth of Tsurumi River Japan. The surface sediment sample was collected by an Ekman-Barge sediment sampler from the Tsurumi-Ohashi bridge in September 1992.
The sediment was black in color indicating sulfate reducing activity.
Macro benthic organisms, such as crabs, small fish and shellfish were present in the collected sediment.
The sediment was sieved through a 2 mm screen to remove debris.
The sieved sediment sample was mixed with river water to make sediment slurry with a solids concentration of 272 +/- 2.8 g/Kg.
This was an average of four replicate measurements.
The pH of the sediment slurry was 5.6.
Duration of test (contact time):
1 yr
Initial test substance concentration
Initial conc.:
4 µmol/L
Based on:
test mat.
Details on study design:
- for each replicate a 5mL of sediment slurry was placed in a screw top test tube and sealed under N2 atmosphere.
- the tubes were sealt and kept at room temperature for one week to ensure that the system was anaerobic.
- all test tubes were placed in an anaerobic chamber (10% H2, 10% CO2, 80% N2)
- 10 µL of stock solution was added to each test tube to give an initial concentration of 4 µmoles/L
- immediately after spiking, time zero (Co) samples were frozen at -20 °C, while other test tubes were kept in a constant temperature room at 25 °C
- the slurries were hand mixed three times a week by turning upside down for few minutes
- the test tubes were sampled once a day at the beginning of the incubation then at longer time intervals as the experiment progressed
- the incubation lasted for a year

Results and discussion

% Degradation
% Degr.:
ca. 98
Parameter:
test mat. analysis
Sampling time:
1 yr
Half-life of parent compound / 50% disappearance time (DT50)
Compartment:
sediment
DT50:
54 d
Type:
not specified
Other kinetic parameters:
first order rate constant
Transformation products:
yes
Identity of transformation productsopen allclose all
No.:
#1
Reference
Reference substance name:
Unnamed
IUPAC name:
3-chloroaniline
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 203-581-0]
CAS number:
108-42-9
Identity:
Benzenamine, 3-chloro-
Molecular formula:
C6H6ClN
Molecular weight:
127.572
SMILES notation:
Nc1cccc(Cl)c1
InChl:
InChI=1/C6H6ClN/c7-5-2-1-3-6(8)4-5/h1-4H,8H2
No.:
#2
Reference
Reference substance name:
Unnamed
IUPAC name:
aniline
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 200-539-3]
CAS number:
62-53-3
Identity:
Benzenamine
Molecular formula:
C6H7N
Molecular weight:
93.126
SMILES notation:
Nc1ccccc1
InChl:
InChI=1/C6H7N/c7-6-4-2-1-3-5-6/h1-5H,7H2

Applicant's summary and conclusion