Registration Dossier

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information


A study was designed to assess the acute toxic effects of the test compound on the zebra fish (Brachydanio rerio HAM. and BUCH.). The study was conducted in compliance with the OECD guideline 203. Based on the nominal concentrations the median lethal concentration LC 50 (96 h) is > 46.4 and < 100 mg/L. Based on the analytically detected concentrations the median lethal concentration LC50 (96 h) is > 41.8 mg/L.

Additionally, in a publication (Edsall 1991) a study conducted on fish larvae (rainbow trout, Oncorhynchus mykiss) is described. 13-21 days old fish were exposed to the test substance in concentrations between 20 and 100 mg/L for 96 hours and mortality recorded. As a result, the LC50 value was determined to be 36.5 mg/L (nominal), but as basic details are missing in this publication, the available data were considered as not reliable.


A study was conducted on daphnia to assess the short-term toxicity of the test substance to aquatic invertebrates. As a result, the EC50 value based on mobility was determined to be 7.29 mg/L.

In a publication (Smith et al. 1988) several substances were tested for their toxicity to Daphnia pulex. The 48 h EC50 of 1-Methylpyrrolidine was determined to be 2.08 mg/L (nominal), based on mobility. In the second publication (Passino & Smith, 1987), a similar study is described revealing an 48 hour EC50 of 4.64 mg/L for the test substance tested with Daphnia pulex.


The growth of green algae exposed to the test item 1-Methylpyrrolidine was investigated in a 72 hour static test according to OECD 201. The endpoint values were determined based on nominal test item concentrations. The EC50 value was determined to be 162.69 mg/L and the EC10 value was 69.07 mg/L.


Respiration Inhibition Test

In a 30 min respiration inhibition test conducted with activated sludge obtained from an industrial wastewater treatment plant, Methylpyrrolidin was found to have an inhibitory effect to respiration. The EC20 was determined to be >100 mg/L.

Microtox assay

In this publication a test is described which was conducted to assess the toxicity of several chemicals including the test substance to different bacteria. Besides evaluating toxicities based on oxygen consumption (aerobic heterotrophs), gas production (methanogens) and Ammonia use (Nitrosomonas), the test substance was also tested in a Microtox test assay (Microbics corporation), based on the biolimunescense of Photobacterium phopsphoreum. As a result, the IC50 value determined in this test system was 8000 mg/L.