Benzenamine, reaction products with aniline hydrochloride and nitrobenzene, hydrochlorides

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Bioaccumulation test in fish performed by MITI.

Qualifier:

according to guideline

Guideline:

other: Bioaccumulation test of a chemical substance in fish or shellfish by MITI

GLP compliance:

not specified

Radiolabelling:

not specified

Test organisms (species):

Cyprinus carpio

Total exposure / uptake duration:

6 wk

Details on test conditions:

RANGE-FINDING / PRELIMINARY STUDY : TLm(48h) > 80 mg/L, Rice fish (Oryzias latipes)

Lipid content:

3.5 %

Type:

BCF

Value:

24 - 49 L/kg

Remarks on result:

other: Conc.in environment / dose:0.8 mg/L

Type:

BCF

Value:

72 - 184 L/kg

Remarks on result:

other: Conc.in environment / dose:0.08 mg/L

Validity criteria fulfilled:

not specified

Conclusions:

The biocconcentration factor (BCF) was determined to be 24-49 L/Kg at an enviornmental concentration of 0.8 mg/L, and 72-184 L/Kg at an environmental concentration of 0.08 mg/L in carp fish (Cyprinus carpio) after 6 week exposure period.

Executive summary:

A bioaccumulation test in fish was performed by MITI. A range finding study was performed on Rice fish (Oryzias latipes) were LC50 after 48 hours exposure was > 80 mg/L. In the main test, carp fish (Cyprinus carpio) were exposed to 0.8 (1st concentration area) and 0.08 mg/L (2nd concentration area) test substance during 6 weeks. The lipid content was 3.5%. The biocconcentration factor (BCF) was determined to be 24-49 L/Kg at an enviornmental concentration of 0.8 mg/L, and 72-184 L/Kg at an environmental concentration of 0.08 mg/L.

Reference 2

Endpoint:

bioaccumulation: aquatic / sediment

Type of information:

calculation (if not (Q)SAR)

Remarks:

Estimated by calculation

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Internationally accepted method, EPI Suite, EPA (USA)

Principles of method if other than guideline:

EPI Suite BCFBAF v3.01

GLP compliance:

no

Test type:

other: estimated by calculation

Details on estimation of bioconcentration:

BASIS FOR CALCULATION OF BCF
- Estimation software: EPI Suite BCFBAF v3.01
- Result based on calculated log Pow of: 9.78

Key result

Type:

BCF

Value:

576 L/kg

Calculation basis:

other: EPI Suite BCFBAF v3.01

Bioaccumulation Estimates (BCFBAF v3.01):

Log BCF from regression-based method = 2.760 (BCF = 576 L/kg wet-wt)

Log Biotransformation Half-life (HL) = -1.7554 days (HL = 0.01756 days)

Log BCF Arnot-Gobas method (upper trophic) = -0.045 (BCF = 0.9021)

Log BAF Arnot-Gobas method (upper trophic) = -0.045 (BAF = 0.9021)

log Kow used: 9.78 (estimated)

Validity criteria fulfilled:

not applicable

Conclusions:

BCF EPISUITE = 576 L/kg wet-wt (BCFBAF v3.01)

Executive summary:

BCF EPISUITE = 576 L/kg wet-wt (BCFBAF v3.01)

Description of key information

Key study: Experimental data. Test performed by MITI. The biocconcentration factor (BCF) was determined to be 24-49 L/Kg at an enviornmental concentration of 0.8 mg/L and 72-184  L/Kg at an environmental concentration of 0.08 mg/L in carp fish (Cyprinus carpio).

Key value for chemical safety assessment

BCF (aquatic species):

184 L/kg ww

Additional information

Key study: Experimental data:

A bioaccumulation test in fish was performed by MITI. Carp fish (Cyprinus carpio) were exposed to 0.8 and 0.08 mg/L test substance during 6 weeks. The lipid content was 3.5%. The biocconcentration factor (BCF) was determined to be 24-49 L/Kg at an enviornmental concentration of 0.8 mg/L, and 72-184 L/Kg at an environmental concentration of 0.08 mg/L in carp fish (Cyprinus carpio) after 6 week exposure period.

Supporting study:

Estimated value for the main component of the substance:

Main component 1: BCF EPISUITE = 576 L/kg wet-wt (BCFBAF v3.01)

The main component of this multiconstituent substance have an extreme log Pow and a high molecular weight and considered as not bioavailable. The components are unlikely to cross biological membranes. Applying Lipinski´s Rule of Five, the substance is not bioavailable and therefore, there is no potential for bioaccumulation.