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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

To evaluate the aquatic toxicity of the test substance studies on fish, aquatic invertebrates algae, duckweed and microorganisms were performed.

Short-term toxicity to fish

Key study:

In a 96 hour acute toxicity study zebrafish (Danio rerio) were exposed to the test item at nominal concentrations of 0 (control), 4.6, 10, 22, 46, 100 mg/L under static conditions in accordance with the OECD 203 test guideline and EU method C.1 Since the measured concentrations of the test substance in the test solutions were within ±20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration for the evaluation of the test substance. The water, pH, temperature and dissolved oxygen were within acceptable guideline specifications. The 96 hour LC50 was determined to be 67.8 mg/L based on the nominal concentration of the test substance.

Supporting study:

The results of this study are supported by a toxicity screening test with fathead minnow (Pimephales promelas) which was conducted using similar methods as described in the OECD 203 test guideline and EU method C.1. The organisms were exposed for 96 hours to the test item in a static test system to concentrations of 0 (control), 1, 10 and 100 mg/L (nominal). The 96 hour LC50 was determined to be within the range of 10 - 100 mg/L.

Short-term toxicity to aquatic invertebrates

Key study:

In a 48-hour acute toxicity study, neonate Daphnia magna were exposed to the test substance at nominal concentrations of 0 (control), 4.6, 10, 22, 46, 100 mg/L under static conditions in accordance with the OECD 202 guideline and EU-method C.2. Since the analytically determined concentrations of the test substance in the test solutions were within +20% of the nominal concentrations in all affected test groups, the effect concentration can be expressed relative to the nominal concentration for the evaluation of the test substance. The water pH, temperature and dissolved oxygen were within acceptable guideline specifications. Immobilization (mortality) and sublethal effects were observed daily.

The 48 hour EC50 value in this acute toxicity study was determined to be > 100 mg/L based on the nominal concentration of the test substance.

Supporting study:

The results of this study are supported by a toxicity screening test with daphnia magna which was conducted using similar methods as described in the OECD 202 test guideline and EU method C.2. The organisms were exposed for 48 hours to the test item in a static test system to concentrations of 0 (control), 1, 10 and 100 mg/L (nominal). The 48 hour EC50 was determined to be > 100 mg/L.

Toxicity to algae

The determination of the inhibitory effect on cell multiplication of the unicellular green algea (desmodesmus subspicatus) was performed with a screening test using similar methods as described in the OECD 201 test guideline and EU method C.3. The organisms were exposed for 72 hours to the test item in a static test system to concentrations of 0 (control), 1, 10 and 100 mg/L (nominal). The 72 hour EC50 was determined to be in the range of 10 - 100 mg/L.

Toxicity to aquatic plants other than algae

The effect of the test item was evaluated in a 7 day growth inhibition test using the aquatic plant Lemna gibba (duckweed). The test was performed according to OECD guideline 221. Following a preliminary non-GLP range-finding test with concentrations 0 (control), 1.00, 10.0 and 100 mg/L a static main test with concentrations of 0 (control), 0.320, 1.00, 3.20, 10.0, 32.0 and 100 was performed. The concentrations in the main test were verified analytically. Since the measured concentrations of the test substance in the test solutions were within ±20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration for the evaluation of the test substance.

Increase of frond numbers, dry weight, growth rate of frond numbers and growth rate of dry weight, yield of frond numbers and yield of dry weight were the parameters for assessment of toxic effects. The EC50 and the NOEC based on growth rate of dry weight was determined to be 10.7 and 3 mg/L (nominal concentration).

 

Toxicity to microorganisms

In a test with domestic activated sludge the inhibition of oxygen consumption was examined according to OECD guideline 209.Bacteria were exposed for three hours to the test substance at concentrations of 62.5, 125, 250, 50, 1000 mg/L. No inhibition of respirometric activity in any of the tested concentrations was detected. Thus the determined EC50 and NOAEC values were > 1000 mg/L.