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Ecotoxicological information

Toxicity to microorganisms

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The toxicity of the registered substance to microorganisms was determined via read-across to the results of testing on the carboxylic acid component of tosyl salt. The highest applied test substance concentration resulted in a total inhibition of ca. 48%. The 3 hour EC50 for respiration inhibition was determined to be >5000 mg/l. 

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The toxicity of the registered substance to microorganisms was determined via read-across to the results of testing on the carboxylic acid component of tosyl salt. The toxicity of tosyl salt to microorganisms was determined in accordance with the OECD Guideline for Testing of Chemicals 209. The highest applied test substance concentration resulted in a total inhibition of ca. 48%. The 3 hour EC50for respiration inhibition was determined to be >5000 mg/l. Read-across between the tosyl salt carboxylic acid (6-[(p-tosyl)amino]hexanoic acid) and the registered substance is considered justified as the registered substance is manufactured directly from 6-[methyl(phenylsulphonyl)amino]hexanoic acid by simple neutralisation with triethanolamine (TEA). 6-[methyl(phenylsulphonyl)amino]hexanoic acid and 6-[(p-tosyl)amino]hexanoic acid are structural isomers. They are the same molecular weight and differ only in the position of a single methyl group. In the former, the methyl group is bound to the nitrogen atom of the sulphonamide linkage whereas in the latter, it resides on the aromatic ring. Other than ionization of the carboxylic acid group, the 6-[methyl(phenylsulphonyl)amino]hexanoic acid remains chemically unchanged upon salt formation. In water, the acid and amine components of 6-[methyl(phenylsulphonyl)amino]hexanoic acid, compound with 2,2’,2’’-nitrilotriethanol (1:1) dissociate completely and behave essentially as independent substances. Since TEA can be considered non-hazardous, it is the acid component of the salt that will have a more significant impact on the outcome of any (eco)toxicological or environmental tests.