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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The 96 hour LC50 of a formulated product containing the registered substance was >1000 mg/l. As no upper limit was provided for the LC50 of the test material it was not possible to accurately calculate the toxicity of the registered substance included in the formulated product. However, as the product contains approximately 50% (44.7%) of the N-methyl salt, and 50% of 1000 mg/l is 500 mg/l it is considered unlikely that the N-methyl salt is toxic to fish.

Key value for chemical safety assessment

LC50 for marine water fish:
1 000 mg/L

Additional information

The acute toxicity of the a formulated product containing the registered substance to fish was determined in accordance with the OECD Guideline for Testing of Chemicals 203. The species used for the test was Scophthalmus maximus (juvenile turbot), which was tested under semi-static conditions for 96 hours. The 96 hour LC50 of the test material was >1000 mg/l. As no upper limit was provided for the LC50 of the test material it was not possible to accurately calculate the toxicity of the registered substance included in the formulated product. However, as the product contains approximately 50% (44.7%) of the N-methyl salt, and 50% of 1000 mg/l is 500 mg/l it is considered unlikely that the N-methyl salt is toxic to fish. In addition, an equivalent study was conducted using a formulated product containing a structurally similar substance (6 -[(p-Tosyl)amino]hexanoic acid, compound with 2,2',2''-nitrilotriethanol (1:1) (Tosyl salt)) to the registered substance. A nominal concentration of 23 mg/l of the test substance was used in the limit test. After 96 hours exposure in this study the LC50 value was determined to be >23 mg/l i.e. higher than the maximum concentration tested. As no upper limit was provided for the LC50 of the test material it was not possible to calculate the toxicity of the registered substance included in the formulated product. Read-across between the registered substance and the test substance (a formulated product containing Tosyl salt) is considered justified owing to the structural similarity of the two substances. Both are salts of a carboxylic acid and triethanolamine. The acid components are structural isomers and differ only in the position of a single methyl group in the N-methyl salt. This is not expected to change the bioavailability of the substances, and both substances have shown similarity in the results of other physical endpoints such as water solubility.