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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: study documented in sufficient detail

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
A test of the equipotency of internal burdens of nine narcotic chemicals using Daphnia magna.
Author:
Pawlisz AV, Peters RH
Year:
1993
Bibliographic source:
Environ Sci Technol 27: 2801-2806
Reference Type:
publication
Title:
Effects of sublethal exposure on lethal body burdens of narcotic organic chemicals in Daphnia magna.
Author:
Pawlisz AV, Peters RH
Year:
1995
Bibliographic source:
Environ Sci Technol 29: 613-621

Materials and methods

Test guidelineopen allclose all
Qualifier:
no guideline available
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
Excessive number of animals per concentration and per test volume. Mortality rather than immobility taken as endpoint.
Principles of method if other than guideline:
Short-term LC50 test with Daphnia magna exposed to acetophenone for 48 h.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): acetophenone
- Substance type: aromatic ketone
- Physical state: liquid (mp: ca. 20°C)
- Analytical purity: >98%
- Impurities (identity and concentrations): not reported

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: not reported
- Sampling method: Acetophenone (radiolabeled) concentration was checked by placing an aliquot of the test solution in a scintillation vial
with Ready Protein scintillation fluid, the measured radioactivity was divided by specfic activity to yield the chemical concentration in mmol/L.
- Sample storage conditions before analysis: not reported

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: An aliquot of the chemical was introduced in a volumetric flask containing standard reconstituted water according to APHA 1989.
- Controls: 2 controls (test substance omitted) performed
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not used
- Evidence of undissolved material (e.g. precipitate, surface film, etc): acetophenone is soluble in water (ws: ca. 6 g/L)

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna (parthenogenic female)
- Strain: not reported
- Source: Biology Department, McGill University 1205 Dr. Penfield Avenue, Montreal, Quebec, H3A 1B1 Canada
- Age at study initiation (mean and range, SD): not reported
- Length at study initiation (length definition, mean, range and SD): 0.4-3.3 mm
- Method of breeding: ca. 75 parthenogenic female were kept in glass beakers (1-L) containing standard reconsituted water acc. to APHA 1989
and under constant environmental conditions (photoperiod: 16 h light/8 h dark, temperature: 20°C). The animals were fed daily,
and each beaker received enough algal suspension to keep the culture water slightly green.
- Feeding during test: no

ACCLIMATION: not reported in respect to the determination of the LC50

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h

Test conditions

Test temperature:
23°C
Nominal and measured concentrations:
nominal
Details on test conditions:
TEST SYSTEM
- Test vessel: eight 20-mL screw-top vials
- Type (delete if not applicable): closed (vials were closed with aluminium-lined screw caps
- Material, size, headspace, fill volume: not reported, 20 mL, 2 mL, 18 mL
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): static test
- No. of organisms per vessel: 15
- No. of vessels per concentration (replicates): 8
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): vehicle not used
- Biomass loading rate: 15 / 18 mL = 0.8 daphnia per mL test medium


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Standard reconstituted water acc. to APHA 1989
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: not reported
- Photoperiod: 16 h light/8h dark (conditions of the stock culture; for the test performance no deviations from these conditions reported)
- Light intensity: not reported

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not reported
- Range finding study: not reported

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
528 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
In a static test, a 48 h LC50=528 mg/L (4.4 mmol/L; nominal) was determined.

Any other information on results incl. tables

In a static test, a 48 h LC50=528 mg/L (4.4 mmol/L; nominal) was determined.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
Acetophenone shows a low acute toxicity towards Daphnia magna (48 h LC50=528 mg/L, nominal).