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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

The EC10 for the bacteria Pseudomonas putida exposed to amides, C8-18 (even numbered) and C18-unsatd., N,N-bis(hydroxyethyl) was 0.83 g/L. The EC50 was 6.0 g/L. Inhibition of cell growth was observed as of 1.0 mg/L. The same data will be used by read across for amides, C12-18 (even numbered) and C18-unsatd., N-(hydroxyethyl). 

Key value for chemical safety assessment

EC50 for microorganisms:
6 g/L
EC10 or NOEC for microorganisms:
0.83 g/L

Additional information

A study was conducted to assess the toxicity of structurally similar amides, C8-18 (even numbered) and C18-unsatd., N,N-bis(hydroxyethyl) to the bacteria Pseudomona putida according to DIN guideline 38412 L8. Pseudomonas putida colonies, in triplicate, were exposed to the test substance at nominal concentrations of 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L. Inhibition of cell growth compared to controls was recorded after 72 hours. The EC10 was 0.83 g/L and the EC50 was 6.0 g/L. Inhibition of cell growth was observed as of 1.0 g/L.