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Diss Factsheets
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EC number: 931-338-5 | CAS number: 90622-77-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From September 08, 1986 to September 11, 1986
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- No analytical dose verification.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- (Pre GLP study; however, GLP comparable quality conditions followed)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: First a stock solution of 10 g of the test substance per litre was prepared in water. From this solution appropriate aliquots were pipetted into the test vessels and filled up to final volumes of 100 mL with mineral nutrient.
- Controls: Nutrient medium with algal culture without test material
- Chemical name of vehicle: Water - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Source (laboratory, culture collection): Pflanzenphysiol, Institut der Universitat Gottingen
- Age of inoculum (at test initiation): A culture of the original delivery was carried on for 6 months
ACCLIMATION
- Acclimation period: Preincubation for about 3-4 d in test medium without test substance - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24.0-25.0°C
- Nominal and measured concentrations:
- 0.3 - 1000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 300 mL conical flasks containing 100 mL liquid volume
- Initial cells density: 1.0E+04 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: Yes (mineral nutrient according to DIN 38412 part 9)
OTHER TEST CONDITIONS
- Light intensity and quality: Continuous uniform illumination with approximately 2,000 lux (daylight-spectrum)
- Stirring: At 500 rpm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Determination of cell concentrations at 24, 48 and 72 h - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: EbC0
- Effect conc.:
- ca. 0.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: EbC50
- Effect conc.:
- ca. 1.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- ca. 8.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Details on results:
- See Table 1 for details.
- Reported statistics and error estimates:
- Probit method was used to determine EbC50 and ErC50 and t-test was used determine EbC0.
- Validity criteria fulfilled:
- not specified
- Remarks:
- (Coefficient of variation for growth rate not reported)
- Conclusions:
- Under the study conditions, 72 h EbC0, EbC50 and ErC50 were found to be 0.3, 1.1 and 8.7 mg/L, respectively.
- Executive summary:
A study was conducted to determine the toxicity of the test substance, C12-18 and C18-unsatd. MEA, to the green algae Scenedesmus subspicatus according to OECD Guideline 201, in compliance with GLP. Algae were exposed to the test substance at concentration ranges of 0.3 to 1000 mg/L for 72 h under static conditions. The algae cell concentrations were determined at 24, 48 and 72 h. The inhibitory effect on algae biomass growth and growth rate was calculated subsequently. No analytical dose verification was conducted. Under the study conditions, the nominal 72 h EbC0, EbC50 and ErC50 were found to be 0.3, 1.1 and 8.7 mg/L, respectively (Gode, 2001).
Reference
Table 1. Cell concentrations and pH values after various time intervals
Substance concentrations (mg product/L) |
Algae cell concentrations (/mL) |
||
Incubation time (h) |
|||
24 |
48 |
72 |
|
0 |
8.4E+04 |
7.2E+05 |
2.3E+06 |
0.3 |
8.2E+04 |
6.7E+05 |
2.0E+06 |
1.0 |
3.9E+04 |
3.3E+05 |
1.3E+06 |
3.0 |
2.2E+04 |
5.8E+04 |
6.2E+05 |
10 |
2.3E+04 |
2.1E+04 |
4.5E+04 |
30 |
1.9E+04 |
2.7E+04 |
2.0E+04 |
100 |
1.0E+04 |
5.0E+03 |
5.0E+03 |
300 |
1.0E+04 |
5.0E+03 |
5.0E+03 |
1000 |
1.0E+04 |
5.0E+03 |
5.0E+03 |
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 8.7 mg/L
- EC10 or NOEC for freshwater algae:
- 0.3 mg/L
Additional information
A study was conducted to determine the toxicity of the test substance, C12-18 and C18-unsatd. MEA, to the green algae Scenedesmus subspicatus according to OECD Guideline 201, in compliance with GLP. Algae were exposed to the test substance at concentration ranges of 0.3 to 1000 mg/L for 72 h under static conditions. The algae cell concentrations were determined at 24, 48 and 72 h. The inhibitory effect on algae biomass growth and growth rate was calculated subsequently. No analytical dose verification was conducted. Under the study conditions, the nominal 72 h EbC0, EbC50 and ErC50 were found to be 0.3, 1.1 and 8.7 mg/L, respectively (Gode, 2001).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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