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EC number: 247-323-5 | CAS number: 25899-50-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Density
- Particle size distribution (Granulometry)
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
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- Acute Toxicity
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- Specific investigations
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- Additional toxicological data

Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- No data
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Relevant methodological deficiencies: male mice only tested, administrated volume was higher than recommended, animals were sacrificed 6 hours after the last administration instead of 18-24 hours.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Deviations:
- yes
- Remarks:
- only males were used, administrated volume was higher than recommended, animals were sacrificed 6 hours after the last administration instead of 18-24 hours.
- Principles of method if other than guideline:
- Method: described in Boller and Schmid (1970) and Schmid (1975)
- GLP compliance:
- no
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- (Z)-pent-2-enenitrile
- EC Number:
- 247-323-5
- EC Name:
- (Z)-pent-2-enenitrile
- Cas Number:
- 25899-50-7
- Molecular formula:
- C5H7N
- IUPAC Name:
- (2Z)-pent-2-enenitrile
- Reference substance name:
- pent-2-enenitrile (Z)
- IUPAC Name:
- pent-2-enenitrile (Z)
- Details on test material:
- - Name of test material (as cited in study report): cis-pentene-2 nitrile commercial
- Physical state: pale yellow liquid
Constituent 1
Constituent 2
Test animals
- Species:
- mouse
- Strain:
- Swiss
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Carworth Farm Lane-Petter
- Age at study initiation: no data
- Weight at study initiation: 25-30 g
- Assigned to test groups randomly: [no/yes, under following basis: ]
- Fasting period before study: no
- Housing: no data
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
No data
IN-LIFE DATES: no data
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle(s)/solvent(s) used: arachis oil
- Justification for choice of solvent/vehicle: no data
- Concentration of test material in vehicle: 0.8 and 4 µL/mL
- Amount of vehicle (if gavage or dermal): 2 x 25 mL/kg - Details on exposure:
- No details
- Duration of treatment / exposure:
- 30 hours
- Frequency of treatment:
- 2 administration (at 0 and 24 hour)
- Post exposure period:
- 6 hours
Doses / concentrations
- Remarks:
- Doses / Concentrations:
2 x 0.02 mL/kg and 2 x 0.1 mL/kg
Basis:
nominal conc.
- No. of animals per sex per dose:
- 10 males per dose
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Benzene (Prolabo, lot n° 79.018)
- Justification for choice of positive control(s): benzene is a recognised clastogenic substance
- Route of administration: oral (gavage)
- Doses / concentrations: 2 x 1.25 mL/kg
Examinations
- Tissues and cell types examined:
- bone marrow (2000 polychromatic erythrocytes per animal)
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION:
Based on a preliminary study.
DETAILS OF SLIDE PREPARATION:
Bone marrow was collected in calf embryo serum. After centrigation, the pellet was homogenized, applied to a slide and coloured with May Grünwald-Giemsa.
METHOD OF ANALYSIS:
1000 polychromatic erythrocytes were counted by 2 persons and a mean value was calculated. - Evaluation criteria:
- % of polychromatic erythrocytes with micronuclei
- Statistics:
- The % of polychromatic erythrocytes with micronuclei in the control and treated groups were compared with the Student t-test.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- not specified
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- RESULTS OF RANGE-FINDING STUDY
- Dose range: 2 x 0.25 and 2 x 0.1 mL/kg
- Clinical signs of toxicity in test animals: At 2 x 0.25 mL/kg, 4/5 animals died. At 2 x 0.1 mL/kg, no mortality was observed, but animals were
prostrated during the hour following the administration.
RESULTS OF DEFINITIVE STUDY
- Induction of micronuclei (for Micronucleus assay): see Table 7.6.2/
- Ratio of PCE/NCE (for Micronucleus assay): no data
- Appropriateness of dose levels and route: no data
- Statistical evaluation: no statistically significant increase in the number of micronuclei was observed in the treated animals.
Any other information on results incl. tables
Table 7.6.2/1: Results of in vivo micronucleus test with cis-pentene-2-nitrile
Group |
Dose |
No. of mice |
% polychromatic erythrocytes with micronuclei |
Control (arachis oil) |
0 |
10 |
0.22 ± 0.08 |
Cis-pentene-2 nitrile com. |
2 x 0.02 mL/kg |
10 |
0.14 ± 0.04 |
Cis-pentene-2 nitrile com. |
2 x 0.1 mL/kg |
10 |
0.13 ± 0.07 |
Benzene |
2 x 1.25 mL/kg |
10 |
3.25 ± 0.72 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
In this study with Cis-pentene-2-nitrile commercial, there was not a significant increase in the frequency of micronucleated polychromatic
erythrocytes in bone marrow after treatment. - Executive summary:
In a Swiss CFLP mouse bone marrow micronucleus assay, 10 males/dose were treated by oral gavage with cis-pentene-2-nitrile commercial (purity unknown) at doses of 0, 2 x 0.02 and 2 x 0.1 mL/kg bw. Bone marrow cells were harvested at 6 hours post-treatment. The vehicle was arachis oil.
Cis-pentene-2-nitrile commercial was tested at an adequate dose (based on a preliminary study results). The positive control induced the appropriate response.There was not a significant increase in the frequency of micronucleated polychromatic erythrocytes in bone marrow after treatment.
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