(2,3-dichlorophenyl)oxoacetonitrile

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study according to international accepted guidelines but not GLP compliant.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

The concentrations of test substance were not analysed in this study. Only nominal concentrations were used.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(2,3-dichlorophenyl)oxoacetonitrile

EC Number:

278-747-9

EC Name:

(2,3-dichlorophenyl)oxoacetonitrile

Cas Number:

77668-42-9

Molecular formula:

C8H3Cl2NO

IUPAC Name:

2,3-dichlorobenzoyl cyanide

Test material form:

solid: crystalline

Details on test material:

Test item: 2,3-DICHLOROBENZOYL NITRILE
CAS No.: 77668-42-9
Batch No.: L36020N
Physical state: Yellow, crystalline powder
Colour: Light-yellow
Purity (GC): 96.48% (based on 2,3-DICHLOROBENZOYL NITRILE content)
Storage 15-30°C

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

The numbers of immobilised daphnids were counted at 24 and 48 hours after the start of test.

Test solutions

Vehicle:

yes

Details on test solutions:

The test organism was breaded and hold in Elendt M4 medium which was used also for preparing of test item stock and working solutions.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Test species: Daphnia magna Straus
Young daphnids were used in the test should have been less than 24 hours old and were not first brood progeny.
Age: < 24 hours neonates
Source: National Institute of Environmental Health

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

21 ± 1 °C

pH:

The pH was measured at the beginning and the end of the test in the control and the group with the highest test substance concentration.

Dissolved oxygen:

The dissolved oxygen was measured at the beginning and the end of the test in the control and the group with the highest test substance concentration.

Nominal and measured concentrations:

The following concentrations were applied: 0.25; 0.50; 1.00; 2.00; 4.00; 8.00 and 16.00 mg/l.
The evaluation of toxic effect was based on the nominal concentrations of test item.

Details on test conditions:

At each concentration level four replicates were used. Each replicate contained 5 daphnids in 75 ml test solution. The test vessels were closed tightly and were kept in thermostat at 21 ± 1 °C with a 16/8 hours light/dark cycle. The treatment of control vessels was same as test vessels.
The pH and oxygen concentrations were measured at the beginning and at the end of test. The numbers of immobilised daphnids were counted at 24 and 48 hours after the start of test.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

1.87 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.03 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL

Details on results:

The statistical analyses of data were performed by ToxRat® software.
The NOEC and LOEC were determined with Fisher’s exact binominal test with Bonferroni correction. The results were inconclusive.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In this Daphnia magna, Acute Immobilisation Test for “2,3-Dichlorobenzoylcyanide” (CAS No. 77668-42-9) the results indicated that the test item is hazardous to the aquatic environment [GHS Acute aquatic hazard Category Acute II. – 48 hr EC50 (for Crustacea) > 1 mg/l but ≤ 10 mg/l]