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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP non-guideline study, available as unpublished report, limitations in design and/or reporting but otherwise adequate for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1973
Report date:
1973

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Before OECD Guideline 404 was establisched in 1982, skin irritation was tested using an internal BASF method. White Vienna rabbits were treated for 1, 5 and 15 minutes and 20 hours using occlusive conditions. An application site of 2.5 x 2.5 cm was covered with 80% aqueous solution of the test substance. In addition, skin tissue from the ear was tested by wrapping the ear. After application time, the skin was washed. The animals were observed 8 days and skin changes were recorded.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,5-dimethylhexyne-2,5-diol
EC Number:
205-533-4
EC Name:
2,5-dimethylhexyne-2,5-diol
Cas Number:
142-30-3
Molecular formula:
C8H14O2
IUPAC Name:
2,5-dimethylhex-3-yne-2,5-diol
Details on test material:
- Name of test material (as cited in study report): Dimethylhexindiol

Test animals

Species:
rabbit
Strain:
Vienna White

Test system

Type of coverage:
occlusive
Preparation of test site:
not specified
Vehicle:
water
Controls:
no
Amount / concentration applied:
An application site of 2.5x2.5 was covered with a 80% aqueous solution
Duration of treatment / exposure:
- back: 1, 5, 15 minutes, and 20 h
- ear: 20 h
Observation period:
8 days
Details on study design:
The skin was washed to remove the test substance.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
mean
Time point:
other: 24 hours
Score:
0
Max. score:
3
Irritation parameter:
edema score
Basis:
mean
Time point:
other: 24 hours
Score:
0
Max. score:
3
Irritant / corrosive response data:
- No effects were observed in animals exposed for 1, 5 or 15 minutes on the back.
- 24 hours after exposure questionable redness was observed in animals exposed on the back for 20 hours. These effects were no longer present 8 days after exposure.
- 24 hours after exposure slight redness was observed in the animals exposed for 20 h on the ear.

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information