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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Biodegradation: 46% (28-d) not readily biodegradable, OECD301B, Harlan Laboratories Ltd. 2013

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Additional information

The ready biodegradability test was carried out according to OECD 301B Ready Biodegradability: CO2 Evolution (Modified Sturm Test) guidelines. The substance, at a concentration of 20 mg Carbon/L, was exposed to activated domestic sewage sludge micro-organisms with mineral medium in sealed culture vessels in the dark at approximately 21 °C for 28 days. The degradation of the test substance was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the reference substance, sodium benzoate, together with a toxicity control were used for validation purposes. The substance attained 46% degradation after 28 days and therefore cannot be considered to be readily biodegradable.