5-chlorovaleryl chloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

5-chlorovaleryl chloride

EC Number:

216-403-1

EC Name:

5-chlorovaleryl chloride

Cas Number:

1575-61-7

Molecular formula:

C5H8Cl2O

IUPAC Name:

5-chloropentanoyl chloride

Details on test material:

- Name of test material (as cited in study report): 5-chloropentanoyl chloride
- Physical state: liquid, colorless
- Analytical purity: 98.5%
- Lot/batch No.: 40/3/Faß 12
- Storage condition of test material: room temperature

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Additional strain / cell type characteristics:

not specified

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Test concentrations with justification for top dose:

20 .0 ug - 5,000 ug/plate (SPT)
62 .5 ug - 1,000 ug/plate (PIT)

Vehicle / solvent:

Vehicle: DMSO

Controls

Controls 1

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene for all strains

Remarks:

with S9 mix

Controls 2

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: N-methyl-N'-nitro-N-nitroso-guanidine (MNNG) for TA100 and TA1535, 4-nitro-o-phenylendiamine for TA98, 9-aminoacridine for TA1537 and N-ethyl-N'-nitro-N-nitrosoguanidin (ENNG) for E.coli

Remarks:

without S9 mix

Details on test system and experimental conditions:

Preincubation test:
0 .1 ml test solution or vehicle, 0 .1 ml bacterial suspension and 0 .5 ml S-9 mix are incubated at 37°C for the duration of 20 minutes . Subsequently, 2 ml of soft agar is added and, after mixing, the samples are poured onto the agar plates within approx . 30 seconds. After incubation at 37°C for 48 - 72 hours in the dark, the bacterial colonies are counted .

Standard Plate test:
0 .1 ml of the overnight cultures is diluted to 10^-6 in each case . Test tubes containing 2 ml portions of soft agar containing maximal amino acid solution (5 mM tryptophan or 5 mM histidine + 0 .5 mM biotin) are kept a water bath at 45°C, and the remaining components are added in the following order :
0 .1 ml vehicle (without and with test substance)
0 .1 ml bacterial suspension (dilution : 10^-6 )
0 .5 ml S-9 mix

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

other: A bacteriotoxic effect was observed from about 1,500 - 2,500 ug/plate onward (standard plate test) or at doses 2 500 μg/late (preincubations test)

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 2

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

other: A bacteriotoxic effect was observed from about 1,500 - 2,500 ug/plate onward (standard plate test) or at doses 2 500 μg/late (preincubations test)

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

No test substance precipitation was found .

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

negative