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EC number: 216-403-1 | CAS number: 1575-61-7
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 5-chlorovaleryl chloride
- EC Number:
- 216-403-1
- EC Name:
- 5-chlorovaleryl chloride
- Cas Number:
- 1575-61-7
- Molecular formula:
- C5H8Cl2O
- IUPAC Name:
- 5-chloropentanoyl chloride
- Details on test material:
- - Name of test material (as cited in study report): 5-chloropentanoyl chloride
- Physical state: liquid, colorless
- Analytical purity: 98.5%
- Lot/batch No.: 40/3/Faß 12
- Storage condition of test material: room temperature
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- E. coli WP2 uvr A
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- 20 .0 ug - 5,000 ug/plate (SPT)
62 .5 ug - 1,000 ug/plate (PIT) - Vehicle / solvent:
- Vehicle: DMSO
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene for all strains
- Remarks:
- with S9 mix
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: N-methyl-N'-nitro-N-nitroso-guanidine (MNNG) for TA100 and TA1535, 4-nitro-o-phenylendiamine for TA98, 9-aminoacridine for TA1537 and N-ethyl-N'-nitro-N-nitrosoguanidin (ENNG) for E.coli
- Remarks:
- without S9 mix
- Details on test system and experimental conditions:
- Preincubation test:
0 .1 ml test solution or vehicle, 0 .1 ml bacterial suspension and 0 .5 ml S-9 mix are incubated at 37°C for the duration of 20 minutes . Subsequently, 2 ml of soft agar is added and, after mixing, the samples are poured onto the agar plates within approx . 30 seconds. After incubation at 37°C for 48 - 72 hours in the dark, the bacterial colonies are counted .
Standard Plate test:
0 .1 ml of the overnight cultures is diluted to 10^-6 in each case . Test tubes containing 2 ml portions of soft agar containing maximal amino acid solution (5 mM tryptophan or 5 mM histidine + 0 .5 mM biotin) are kept a water bath at 45°C, and the remaining components are added in the following order :
0 .1 ml vehicle (without and with test substance)
0 .1 ml bacterial suspension (dilution : 10^-6 )
0 .5 ml S-9 mix
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: A bacteriotoxic effect was observed from about 1,500 - 2,500 ug/plate onward (standard plate test) or at doses 2 500 μg/late (preincubations test)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: A bacteriotoxic effect was observed from about 1,500 - 2,500 ug/plate onward (standard plate test) or at doses 2 500 μg/late (preincubations test)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- No test substance precipitation was found .
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- negative
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