5-chlorovaleryl chloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method B.1 (Acute Toxicity (Oral))

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

5-chlorovaleryl chloride

EC Number:

216-403-1

EC Name:

5-chlorovaleryl chloride

Cas Number:

1575-61-7

Molecular formula:

C5H8Cl2O

IUPAC Name:

5-chloropentanoyl chloride

Details on test material:

- Name of test material (as cited in study report): 5-chloropentanoyl chloride
- Physical state: liquid, achromatic
- Storage: room temperature

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. K. Thomae GmbH, Biberach, FRG
- Age at study initiation: young adults
- Weight at study initiation: Males: 168 - 187 g; Females: 173 - 181 g
- Fasting period before study: at least 16 hours, but water remained available
- Housing: single housing, in stainless steel wire mesh cages, type DK-III (Becker & Co., Castrop-Rauxel, FRG); no bedding in cages, sawdust in the waste trays.
- Diet: ad libitum, Kliba-labordiaet 343, Klingentalmuehle AG Kaiseraugst, Switzerland
- Water: ad libitum, tap water
- Acclimation period: at least 1 week

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 24
- Humidity (%): 30 - 70
- Air: fully air-conditioned rooms
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Remarks:

DAB 10

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 4, 10 and 40 g/100 mL
- Administration volume: 5 mL/kg
- Justification for choice of vehicle: test substance is sensitive to hydrolysis

CLASS METHOD
- Rationale for the selection of the starting dose: A starting dose of 200 mg/kg bw was chosen based on the physical and chemical characteristics of the test substance and the composition.

Doses:

Group 1: 200 mg/kg bw
Group 2: 500 mg/kg bw
Group 3: 2000 mg/kg bw

No. of animals per sex per dose:

Group 1: 3 males & 3 females
Group 2: 3 males & 3 females
Group 3: 3 males

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days for group 1 and 2, 1 hour for group 3
- Frequency of observations and weighing: Recordings of signs and symptoms several times on the day of administration, at least once each workday for the individual animals. Individual body weights shortly before application (day 0), weekly thereafter and at the end of the study (before fasting period). A check for any dead or moribund animal was made twice each workday and once on Saturdays, Sundays and on public holidays.
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 500 - <= 2 000 mg/kg bw

Based on:

test mat.

Mortality:

- No mortality was observed in animals exposed to 200 or 500 mg/kg bw.
- All animals exposed to 2000 mg/kg died.

Clinical signs:

other: - No clinical signs were observed in female animals exposed to 200 mg/kg bw. - An impaired general state, dypnoea, staggering and piloerection were observed in all female animals exposed to 500 mg/kg bw, between 0 and 5 hours after exposure. - A poor gene

Gross pathology:

- Animals that died: A bad postmortal state was observed.
- Sacrificed animals: no pathologic findings were noted.

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information