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EC number: 222-294-1 | CAS number: 3407-42-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed using standard test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Principles of method if other than guideline:
- Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the test substance according to OECD Guideline 201.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- Acetone
- Details on test solutions:
- The stock solution was prepared by dissolving colourless dense liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available
ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed: - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- ±1 hour
- Test temperature:
- 23±2°C
- pH:
- Without adjustment, the sample at test concentration 68 mg/l : pH = 8.2 changed to pH = 8.1 during the test.
Control: 8.2 (changed to 8.6 during test) - Nominal and measured concentrations:
- 0, 2, 4, 8.5, 16, 32 and 68 mg/L nominal test concentrations
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 30 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3
- Other:
Control solution: OECD growth medium without the sample, the coefficient of variation of average growth rate in replicates during the whole test period was 0.5% without acetone and 0.3% with acetone.
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- 47 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 37.3 - 59.1
- Results with reference substance (positive control):
- Results with reference substance valid
- ErC50: 0.65 mg/L - Reported statistics and error estimates:
- ErC50 was calculated using non linear regression by the software Prism 4.0
- Validity criteria fulfilled:
- yes
- Conclusions:
- The median effective concentration (ErC50) of the test substance on Desmodesmus subspicatus was determined to be 47.0 mg/L on the basis of effects on growth rate of algae in a 72 hour study.
- Executive summary:
Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance according to the OECD Guideline 201. The stock solution was prepared by dissolving colourless dense liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study were 0, 2, 4, 8.5, 16, 32 and 68 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (ErC50) of the test substance on green algae Desmodesmus subspicatus was determined to be 47.0 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP classification criteria.
Reference
Description of key information
Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance according to the OECD Guideline 201. The stock solution (32 g/L) was prepared by dissolving colourless dense liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study were 0, 2, 4, 8.5, 16, 32 and 68 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (ErC50) for the test substance in Desmodesmus subspicatus was determined to be 47.0 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP classification criteria.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 47 mg/L
Additional information
Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance according to the OECD Guideline 201. The stock solution (32 g/L) was prepared by dissolving colourless dense liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study were 0, 2, 4, 8.5, 16, 32 and 68 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (ErC50) for the test substance in Desmodesmus subspicatus was determined to be 47.0 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP classification criteria.
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