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Ecotoxicological information

Toxicity to terrestrial plants

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Administrative data

Endpoint:
toxicity to terrestrial plants: short-term
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study peer reviewed in EU-Risk assessment

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Phytotoxicity studies with Lactuca Sativa in soil and nutrient solution
Author:
Hulzebos et al.
Year:
1993
Bibliographic source:
Environ. Toxicol. Chem. 12(6), 1079-1094
Reference Type:
secondary source
Title:
3,4-dichloroaniline (3,4-DCA)
Author:
EU-Risk Assessment Report
Year:
2006
Bibliographic source:
EUR 22235 EN; ISSN 1018-5593

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
3,4-dichloroaniline
EC Number:
202-448-4
EC Name:
3,4-dichloroaniline
Cas Number:
95-76-1
Molecular formula:
C6H5Cl2N
IUPAC Name:
3,4-dichloroaniline
Details on test material:
- Name of test material (as cited in study report): 3,4-Dichloranilin,
- Purity: >= 95 %

Sampling and analysis

Analytical monitoring:
yes

Test substrate

Vehicle:
no

Test organisms

Species:
Lactuca sativa
Plant group:
Dicotyledonae (dicots)

Study design

Test type:
early seedling growth toxicity test
Study type:
laboratory study
Substrate type:
natural soil
Limit test:
no
Total exposure duration:
14 d

Test conditions

Test temperature:
21 +/- 4 °C
pH:
7.5
Nominal and measured concentrations:
Nominal concentrations from 0.32 to 100 mg/kg dw soil were spaced by a factor of 3.2.

Results and discussion

Effect concentrationsopen allclose all
Species:
Lactuca sativa
Duration:
7 d
Dose descriptor:
EC50
Effect conc.:
> 10 - < 32 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Lactuca sativa
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
> 10 - < 32 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Lactuca sativa
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
1.7 other: mg/L nutrient solution
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: Day 16 and 21

Any other information on results incl. tables

The test was performed according OECD Guideline 208. Nominal concentrations from 0.32 to

100 mg/kg dw soil were spaced by a factor of 3.2. Duplicate trays were used for the control and

at least three test concentrations. Immediately after DCA treatment seeds were put into the soil.

After four days, only the first five germinated seedlings were required, and any additional

seedlings were discarded in order to exclude the effect of the binding of 3,4-DCA to soil

organics. Seeds could possibly have germinated after four days as 3,4-DCA was less available

and this effect was outside of the scope of this test. After 7 and 14 days, shoots were harvested

by cutting them off at soil level. The fresh weight of each plant was determined immediately

after harvesting. Water content and pH of the soil were checked at the start and at the end of the

experiment. The concentration of 3,4-DCA was analysed by extraction with toluene, followed by

analysis of the extract. The concentration of 3,4-DCA has dropped to a low value (< 30%) of the

initial concentration. The EC50 obtained for 3,4-DCA is slightly above 10 mg/kg soil dw. One

concentration higher (32 mg/kg dry soil), seeds did not germinate, and therefore it is set equal to

10.

Additionally the result in nutrient solution test:

16-21-day EC50 = 1.7 mg/l nutrient solution

Effect: growth (fresh weight), organic matter 1.4%, pH 7.5, nominal concentration.

Applicant's summary and conclusion