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Based on the results from an earthworm reproduction toxicity test according to OECD 222 and GLP, no acute and chronic toxicity were observed. The 28 -d toxicity to adult earthworms was > 1000 mg/kg dw. The 56 -d NOEC for reproduction was determined to be > = 1000 mg/kg dw.


The substance showed also no effect on soil microorganisms in a nitrogen transformation study according to OECD 216 and GLP. The EC10 was determined to be >= 1000 mg/kg dw after 28 days. Because the degree of inhibition at 1000 mg/kg dw was less than 25 % at the end of the exposure, Tris-(2-ethylhexyl)amine can be evaluated as having no long-term influence on nitrogen transformation in soils.


The chronic toxicity to terrestrial plants has been studied in two GLP OECD 208 guideline studies with six plant species. Triticum aestivum proved to be the most sensitive species with a NOEC of 7.8 mg/kg dw (shoot-length). This value indicates a potential for long-term effects of the substance. However, the substance is not supposed to be directly applied to soil. The risk characterisation ratio (RCR < 1) does not indicate the need for further investigation of risks for the soil compartment.