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Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

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Administrative data

Endpoint:
biodegradation in water: simulation testing on ultimate degradation in surface water
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1980-1981
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented publication which meets basic scientific principles. (referring to Bartholomew and Pfaender, 1983)

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Influence of spatial and temporal variations on organic pollutant biodegradation rates in an estuarine environment.
Author:
Bartholomew GW and Pfaender FK
Year:
1983
Bibliographic source:
Applied and Environmental Microbiology, Vol. 45, No. 1, 103-109
Reference Type:
secondary source
Title:
1,2,4-Trichlorbenzol - BUA-Stoffbericht 17 - (Dezember 1987) [Report in German]
Author:
Beratergremium für umweltrelevante Altstoffe (BUA)
Year:
1987
Bibliographic source:
Wissenschaftliche Verlagsgesellschaft VCH, Weinheim, ISSN: 0179-2601

Materials and methods

Principles of method if other than guideline:
Biodegradation rates were assessed by heterotrophic uptake kinetics (Pfaender and Bartholomew, 1982, Appl. Environ. Microbiol. 44:159-164). This protocol measures both the uptake of radiolabeled substrate into microbial cells and subsequent respiration to 14CO2. After incubation, the 14CO2 produced from respiration was trapped from the headspace above acidified samples, and substrate uptake into cells was determined by filtering the water sample through membrane filters.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1,2,4-trichlorobenzene
- Analytical purity: no data
- Specific activity (if radiolabelling): 13.1 mCi/mmol
- Locations of the label (if radiolabelling): 1,2,4-trichloro[U-14C]benzene
Radiolabelling:
yes

Study design

Inoculum or test system:
natural water
Duration of test (contact time):
>= 4 - <= 10 h

Results and discussion

Any other information on results incl. tables

summary of kinetic parameters for 1,2,4-trichlorobenzene

Date

Site

Temperature

[°C]

Vmaxa)

[ng * l-1* h-1]

Kmb)

 

11-7-80

Upstream

12

7.5

6.5

Estuarine

14

<1

-

Marine

17

<1

-

 

5-26-81

Upstream

24

<1

-

Estuarine

28

7.9

7.0

Marine

24

2.3

4.5

a) susbstrate uptake into cells. A Vmax< 1 ng * l-1* h-1represents a metabolic rate less than that detectable by this technique.

b) CO2production from respiration

Applicant's summary and conclusion