2,4'-difluorobenzophenone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006.07.10-2006.07.14

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DS/EN ISO 14669

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,4'-difluorobenzophenone

EC Number:

206-441-7

EC Name:

2,4'-difluorobenzophenone

Cas Number:

342-25-6

Molecular formula:

C13H8F2O

IUPAC Name:

(2-fluorophenyl)(4-fluorophenyl)methanone

Details on test material:

- Name of test material (as cited in study report):2,4-difluorobenzophenone
- Physical state: liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: (Water accomodated fraction (WAF)): 10, 26, 64, 160, 400 and 1000 ml WAF/l
- Sampling method: 2 x 50 ml of stock solution
- Sample storage conditions before analysis: Freezer at -21ºC

Test organisms

Test organisms (species):

other: Arcartia tonsa (DANA)

Details on test organisms:

TEST ORGANISM
- Common name: Marine copepod

ACCLIMATION
- Acclimation conditions : no acclimatation needed, test done under same conditions at same facility as test animals was optained from

Study design

Test type:

semi-static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20 ±0.5 ˚C

pH:

7.8

Salinity:

32 ‰

Nominal and measured concentrations:

Stock solution made by 500 mg substance sissolved in 500 ml test media. Nominal concentration: 1000 mg/l.
Substance was not fully dissolved, therfore a test was taken from the middle of the volumetric flask (water accomodated fraction = WAF)

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 ml Pyrex glass beakers
- Fill volume: 25 ml test solution
- Renoval: After 24 h 25 ml test solution was added
- No. of organisms per vessel: 5-7
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 5-6
- No. of vessels per vehicle control (replicates): 6

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol, CAS No. : 591-35-5

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

4.83 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

mortality

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Mortality: 50% at 1.57 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Mortality in controls < 10 %, oxygen saturation ≥ 4 mg/l, mortality with reference test within acceptable range, 0.34-1.68 mg/l %

Conclusions:

LC50(48 h) of 2,4'-difluorobenzophenone, CAS No. 342-25-6 is 4.83 mg/L for Arcartia tonsa