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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DS/EN ISO 14669 (1999)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: Liquid
Details on test material:
- Analytical purity: 98 % w/w

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations:(nominial) 0, 2, 5, 10, 20, 50, 100 and 200 mg/l

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
other aquatic crustacea: Acartia tonsa
Details on test organisms:
TEST ORGANISM
- Common name: Calanoid copepod (marine)
- Strain: Kept in culture at DHI since 1987, originally collected in the North Sea by Danmarks Fiskeriundersøgelser

Study design

Test type:
static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
19 ± 0.5 ºC
pH:
8.0 ± 0.2
Dissolved oxygen:
≥ 4 mg/l at test end
Salinity:
33 ‰
Nominal and measured concentrations:
Nominal concentrations: 0, 2, 5, 10, 20, 50, 100 and 200 mg/
Details on test conditions:
TEST SYSTEM
- Test vessels: 50 ml glass beakers with approx. 25 ml of test soulution

- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates):


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater, filtered, collected in the North Sea near Hirtshals by Danmarks Fiskeriundersøgelser 26.10.2009
-

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 (light:dark)
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol, CAS No. : 591-35-5

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
ca. 105 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC10
Effect conc.:
> 10 - < 20 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Mortality: yes
- LC50: 1.64 mg/l (a little higher than validation criterium, but not considered to have had significant influence on the test systems sensitivity

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
LC50(48 h) of O,O-diisopropyl hydrogen dithiophosphate, CAS No.107-56-2, is 105 mg/L for Acatia tonsa
Control group mortality was a little higher than validation criterium, but this is not considered to have had significant influence on the test result.