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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
other: Protocols on Methods for the Testing of Chemicals Used in the Offshore Industry (1995) Part B. Protocol for a Fish Test Acute Toxicity Test
Deviations:
yes
Remarks:
Slightly bigger fish was used resulting in a weight load per test container volume(3.2-3.45 g/l) than recomended in guideline (1 g/l). This is considered to be of minor importance.
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
Modified according to Protocols on Methods for the Testing of Chemicals Used in the Offshore Industry (1995) Part B.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
- Analytical purity: 98 % w/w

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
- Concentrations:(nominial) 0, 10, 20, 50, 100 and 200 mg/l
- Sampling method: 40 ml taken out in 50 ml bottles of all testkoncentrations
- Sample storage conditions before analysis: Frozen at - 20 C right after sampling

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
other: Scophtalmus maximus
Details on test organisms:
TEST ORGANISM
- Common name: Turbot
- Source: Maximus in Thisted, Denmark
- Age at study initiation (mean and range, SD): Juvenile

Study design

Test type:
static
Water media type:
saltwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Test temperature:
14.5 ± 0.5 ºC
pH:
7.9 - 0.1
Dissolved oxygen:
>70 %
Salinity:
33 ‰
Nominal and measured concentrations:
Nominal concentrations: 0(control), 0, 10, 20, 50, 100 and 200 mg/l.
Details on test conditions:
- Test vessels: 20 l aquariums

- No. of organisms per vessel: 5

- Biomass loading rate: approx 3-4 g per l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 12:12 (light:dark)

Reference substance (positive control):
yes
Remarks:
Potassium dichromate, CAS No. : 7778-50-9

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC10
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Mortality: yes
- LC50: 300-500 mg/l
- Other:

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
LC50(96 h) of O,O-diisopropyl hydrogen dithiophosphate, CAS No.107-56-2, is above 200 mg/L for Scophtalmus maximus