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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The test item was investigated for its ready biodegradability in a CO2 Evolution test. The test item was not degraded within 28 days. The test item is considered to be not readily biodegradable. 

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

CO2 Evolution test

A study was performed to determine the potential for ready biodegradation of the test item in water by the carbon dioxide (CO2) evolution method following OECD 301B, adopted in 1992. The CO2 released upon biodegradation of the test item and the reference substance sodium benzoate was measured over 28 days. Two blank controls (containing only inoculum) and one toxicity control (containing inoculum, reference and test substances) were established to account for background CO2 production and toxicity of the test item, respectively. All validation criteria were fulfilled. The sodium benzoate controls had degraded > 60 % at day 8. Based on the CO2 analysis, there was no biodegradation observed for the test item within the 28 days. The degradation of the test item was therefore less than the minimal guideline demand of 60 %. Therefore the test item is considered to be not readily biodegradable.