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Environmental fate & pathways

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Ready biodegradation

A study was performed to determine the potential for ready biodegradation of the test item by the carbon dioxide (CO2) evolution method following OECD 301B, adopted in 1992. The CO2 released upon biodegradation of the test item and the reference substance sodium benzoate was measured. Two blank controls (containing only inoculum) and a toxicity control (containing inoculum, reference and test substances) were established to account for background CO2 production and toxicity of the test item, respectively. All validation criteria were fulfilled. The sodium benzoate controls had degraded > 60 % at day 8. Based on the CO2 analysis, there was no biodegradation observed for the test item within the 28 days. The degradation of the test item was therefore less than the minimal guideline demand of 60 %.Therefore the test item is considered to be not readily biodegradable.

Biodegradation in water and sediment: simulation tests

Biodegradation in soil

Direct and indirect exposure of the test item to water, sediment and soil is unlikely. The test substance is practically insoluble in water. In addition, low exposure is guaranteed, as no sludge application from STP on soil will be done and aerial deposition is negligible. Thus, simulation testing for biodegradation in water, sediment and soil was considered not scientifically justified.