Zeolite, phosphor containing, crystalline, synthetic, non fibrous

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

2005-01 to 2005-02

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP-Guideline study. According to the ECHA guidance document "Practical guide 6: How to report read-across and categories (March 2010)", the reliability was changed from RL1 to RL2.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes (incl. QA statement)

Remarks:

Landesamt für Umweltschutz und Gewerbeaufsicht, Rheinland-Pfalz, Germany

Test material

Test material information

Constituent 1

Reference substance name:

Automatically generated during migration to IUCLID 6, no data available

IUPAC Name:

Automatically generated during migration to IUCLID 6, no data available

Details on test material:

- Name of test material (as cited in study report): Ca++-loaded Zeolite X/A (80/20%)
- Chemical name: Zeolite, cuboidal, crystalline, synthetic, non-fibrous
- Framework: cuboidal
- Related CAS number: 1318-02-1
- Analytical purity: 100%
- Lot/batch No.: 9323
- Expiration date of the lot/batch: 31.12.2006
- Chemical name: Ca-Aluminosilicate
- Storage condition of test material: room temperature
- surface modified with Ca

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

Since the test substance is poorly water soluble, a saturated solution was prepared by shaking a nominal load of 1000 mg/L with deionized water for 24 hours, followed by membrane filtration.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Newborn daphnia (strain: STRAUS, aged 0 to 23 hours, bred in the LAUS GmbH according to EN ISO 6341, originally obtained from Federal Environment Agency Berlin) were transferred to M4 Elendt medium for a settling-in period of 30 minutes.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Hardness:

157-283 mg CaCO3/L

Test temperature:

19.7 to 22.4°C

pH:

7.7 to 8.5

Dissolved oxygen:

7.7 to 9.4 mg/L

Nominal and measured concentrations:

0, 10, 32, 100, 320 and 1000 mg/L (nominal)

Details on test conditions:

Newborn daphnia (aged 0 to 23 hours, bred in the LAUS GmbH according to EN ISO 6341, originally obtained from Federal Environment Agency Berlin) were transferred to M4 Elendt medium for a settling-in period of 30 minutes. Undamaged individuals were caught using a glass tube (diameter 8 mm) and put into the respective test vessel (glass beakers, 100 mL) containing the test solution (loading 80 mL/animal, animals were held individually, 10 replicates/treatment, no aeration).
Following nominal concentrations were tested: 0, 10, 32, 100, 320 and 1000 mg/L (dilution water: tap water, TOC 0.82 mg/L, pH 8.17, conductivity 249 µS/cm, hardness 0.943 mmol/L; enriched to M4 Elendt medium).
Test vessels were inspected for offspring daily. Newborn daphnids were counted and removed from the test vessel.
Medium renewal was 3 x per week using freshly prepared test solutions.
pH and oxygen were measured in both the old and the new test solutions, hardness was determined once a week.
Daphnids were fed with Desmodesmus subspicatus (0.15-0.20 mg org. C/animal/day).
Statistical significance was tested by means of t-test or Weir-test, if homogeneity of variances was not given. EC50 was calculated according to a sigmoidal fit of the data (OriginTM).

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

eluate

Basis for effect:

reproduction

Effect concentrations 2

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

eluate

Basis for effect:

reproduction

Effect concentrations 3

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

680 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

eluate

Basis for effect:

reproduction

Details on results:

Temperature during the test was 19.7 to 22.4°C, pH was 7.7 to 8.5, hardness was 157-283 mg CaCO3/L and O2 content was 7.7 to 9.4 mg/L. Lighting was below 2000 lux.
The NOEC was 32 mg/L, the corresponding LOEC 100 mg/L and the EC50 680 mg/L, based on nominal concentrations.
Mortality in the control was 0%, mean offspring was 96.
Following mean offspring numbers (in brackets inhibition of reproduction compared to the control) were obtained for the tested concentrations: 10 mg/L 104 (-9%), 32 mg/L 100 (-5%), 100 mg/L 74 (22%), 320 mg/L 82 (14%) and 1000 mg/L 30 (69%).
Mortality was 10% in the 320 mg/L treatment and 40% in the 1000 mg/L treatment. Results for the treatments 100 and 1000 mg/L were statistically significant (p<0.01).

Any other information on results incl. tables

Summary of results:

Treatment (mg/L)	Total offspring	Surviving parent animals	Offspring/ surviving parent animal	Inhibition of reproduction (%)
control	958	10	96	0
10	1043	10	104	-9
32	1003	10	100	-5
100	743	10	74	22
320	739	9	82	14
1000	177	6	30	69