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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: In accordance with current guidelines

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1981
Report date:
1981

Materials and methods

Principles of method if other than guideline:
Method: other: see freetext ME
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
4-chlorophenyl isocyanate
EC Number:
203-176-9
EC Name:
4-chlorophenyl isocyanate
Cas Number:
104-12-1
Molecular formula:
C7H4ClNO
IUPAC Name:
1-chloro-4-isocyanatobenzene
Details on test material:
IUCLID4 Test substance: other TS: 4-chlorophenyl isocyanate, purity: >99%

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
nose only
Vehicle:
other: air
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
47, 113, 272, 255, 422 mg/m³/4h
No. of animals per sex per dose:
10
Control animals:
not specified

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LC50
Effect level:
>= 113 - <= 272 mg/m³ air
Exp. duration:
4 h
Sex:
female
Dose descriptor:
LC50
Effect level:
ca. 113 mg/m³ air
Exp. duration:
4 h
Mortality:
no data
Clinical signs:
other: no data
Body weight:
no data
Gross pathology:
no data
Other findings:
no data

Any other information on results incl. tables

---Signs of intoxication:
All animals showed signs of intoxications including
unspecific disturbance of behaviour and difficulties in
breathing. These symptoms could be observed up to the end of
the observation period. In addition, irritational effects on
visible mucous membranes of nose and eyes and increased
lacrimation and salivation were noted. Animals of the
highest concentration group lost body weight. 

---Mortality
Concentration---males//females---time of death: m.//fem.
 47 mg/m³--- 1/10 // 0/10 --- 11 d //  --
113 mg/m³--- 2/10 // 5/10 ---12-14 // 10-15 d 
272 mg/m³--- 8/10 // 7/10 --- 3-17 //  2-15 d
255 mg/m³---10/10 // 9/10 --- 4-16 //  1-13 d
422 mg/m³---10/10 //10/10 --- 1-9  //  2-15 d

---Value: 
male: 0.113 - 0.272 mg/l; 
female:  ca. 0.113 mg/l;

---Necropsy:
distended lungs, pale coloured with dark red areas, pleural
fluid, pale kidneys and spleen

Applicant's summary and conclusion

Executive summary:

method: rats were exposed nose-only to the test substance for 4 hours and then observed over 21 days for signs of intoxication and mortality. 

Body weight was recorded and necropsy performed.

result: LD50 = 113 - 272 mg/m³ (male rats) and ca. 113 mg/m³ (female rats).

All animals showed signs of intoxications including
unspecific disturbance of behaviour and difficulties in
breathing. These symptoms could be observed up to the end of
the observation period. In addition, irritational effects on
visible mucous membranes of nose and eyes and increased
lacrimation and salivation were noted. Animals of the
highest concentration group lost body weight.