4-chlorophenyl isocyanate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: In compliance with current guideline, but individual animal data were not shown

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Principles of method if other than guideline:

5 rats/sex/dose group,  test substance was diluted in polyethylene glycol 400, single application by gavage after rats had fasted for 20-24 hours. Symptoms and mortality were recorded for 14 days. Dead rats and survivors were necropsied and grossly examined by a general inspection of digestive tract, liver, lungs, kidneys, heart and spleen. LD50 was calculated according to the method of Weil (1952).

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-chlorophenyl isocyanate

EC Number:

203-176-9

EC Name:

4-chlorophenyl isocyanate

Cas Number:

104-12-1

Molecular formula:

C7H4ClNO

IUPAC Name:

1-chloro-4-isocyanatobenzene

Details on test material:

IUCLID4 Test substance: other TS: p-chlorophenyl isocyanate, no data on purity

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

polyethylene glycol

Doses:

male: 168, 286, 486 and 826 mg/kg bw; female: 58, 99, 168, 286 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

not specified

Statistics:

LD50 was calculated according to the method of Weil (1952).

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

335 mg/kg bw

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

138 mg/kg bw

Mortality:

rats (male); deaths/symptoms/no exposed:
168 mg/kg 0/5/5; 286 mg/kg 3/5/5; 486 mg/kg 3/5/5; 826 mg/kg 5/5/5;
rats (female); deaths/symptoms/no exposed:
58 mg/kg 0/5/5; 99 mg/kg 2/5/5; 168 mg/kg 3/5/5; 286 mg/kg 4/5/5;

Clinical signs:

other: lethargy was the only sign of intoxination preceding death

Gross pathology:

a gross necropsy was conducted with a general inspection of the digestive tract, liver, lungs, kidneys, heart and spleen

Any other information on results incl. tables

RS-Freetext:
Dose        deaths/exposed   time of death     
(mg/kg bw)   male//female     (hours)

   58             // 0/5           // --         
   99             // 2/5           // 3
  168         0/5 // 3/5       --  // up to 24
  286         3/5 // 4/5       2.5 // 2.5
  486         3/5 //           1.5 //
  826         5/5 //           1   //

LD50 value: male: 355 mg/kg bw; female: 138 mg/kg bw

Signs of intoxication were observed in all rats in test and
included lethargy.
Gross necropsy revealed gastrointestinal inflammation in
dead rats; internal organs of survivors appeared normal.

Applicant's summary and conclusion

Executive summary:

5 rats/sex/dose group,  test substance was diluted in polyethylene glycol 400, single application by gavage after rats had fasted for 20-24 hours. Symptoms and mortality were recorded for 14 days. Dead rats and survivors were necropsied and grossly examined by a general inspection of digestive tract, liver, lungs, kidneys, heart and spleen.

LD50 value: male: 355 mg/kg bw; female: 138 mg/kg bw.

Gross necropsy revealed gastrointestinal inflammation in
dead rats; internal organs of survivors appeared normal.

Lethargy was the only sign of intoxination preceding death.