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EC number: 230-363-2 | CAS number: 7069-42-3
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study conducted in accordance with GLP, however no analytical determination of the test substance was performed.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: German national standard DIN 38 412, part L15
- GLP compliance:
- no
Test material
- Reference substance name:
- Retinyl propionate
- EC Number:
- 230-363-2
- EC Name:
- Retinyl propionate
- Cas Number:
- 7069-42-3
- Molecular formula:
- C23H34O2
- IUPAC Name:
- retinyl propionate
- Details on test material:
- - Vitamin A-propionat 2.5 Mio.
- Identification Code: 04-4097-3
- Purity: approx. 95 %
- Batch No.: 6269, ABLAGE-NR. 80-7031
- Water solubility: insoluble (according to sponsor)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test solution was prepared by adding the test item to the test media without any pre-treatment. Subsequently the fish were placed into the test aquaria
Test organisms
- Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM
- Common name: Golden orfe
- Supplier: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Body length: 6.1 cm (range 5.7 - 6.6 cm)
- Body weight: 2.8 g (range 2.4 - 3.9 g)
- Corpulence factor of the test fish batch: 1.26
HOUSING AND ACCLIMATION
- Culture conditions: oil-free aerated and charcoal filtered tap water, flow-through system
- Water temperature: 15 - 20 °C
- Duration of housing: about 3 months
- Mortality during the last 2 weeks of housing: 0.03 %
- Mortality during the adaption period: 0 %
- Medical treatment: twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride
- Diet: Growing feed F/B 50, ad libitum
- Adaption to test water and test temperature: 3 days
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 2.5 mmol/L
- Test temperature:
- 21 °C
- pH:
- 7.7 - 8.2
- Dissolved oxygen:
- 7.3 - 8.5 mg/L
- Nominal and measured concentrations:
- Nominal test concentrations: 5000 and 10000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessels: All glass aquaria (30 x 22 x 24 cm)
- Volume of water: 10
- No of animals per test concentration and control: 10
- Loading rate (g fish/l test water): 2.8
- Aeration: slight
- Withdrawal of food: 1 day before and during exposure
TEST MEDIUM / WATER PARAMETERS
- Test water: reconstituted freshwater according to DIN 38412, Part II, 1982; prepared from fully demineralised tap water (conductivity: max 10 micro MHO) by adding: 294.0 mg/L CaCl2 * 2 H2O, 123.3 mg/L MgSO4 + 7 H2O, 63.0 mg/L NaHCO3, 5.5 mg/L KCl
- Aeration:Continuous aeration with oil-free air
- Acid capacity: 0.8 mmol/l
- Ratio Ca/Mg ions: 4 : 1
- Ratio Na/K ions: 10 : 1
- pH: about 8.0
OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime
EFFECT PARAMETERS MEASURED: mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 h
TEST CONCENTRATIONS
- Test concentrations based on the results of a range-finding test
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- - During the 96 h exposure to Vitamin A propionate no mortalities or symptoms of intoxication were detected.
- No analytical dose-verification of the test substance was carried out.
- Oily, drop-shaped test substance was visible on the water surface
Applicant's summary and conclusion
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