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Environmental fate & pathways

Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
18 January 2011 to 01 June 2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of method:
HPLC estimation method
Media:
soil
Radiolabelling:
no
Details on study design: HPLC method:
EQUIPMENT
- Apparatus: Agilent 1200 Series Liquid Chromatograph
- Type, material and dimension of analytical (guard) column: HyperClone CN (CPS) (25 cm x 4.6 mm internal diameter)
- Detection system: UV set at 210 nm (reference mixture), Refractive Index (samples)

MOBILE PHASES
- Type: Methanol:water (55:45 v/v)
- Experiments with additives carried out on separate columns: no

DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column

REFERENCE SUBSTANCES
- Identity: Acetanilide, Nitrobenzene, Naphthalene, 2-Methylnaphthalene, Anthracene, DDT

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: no data
- Quantity of reference substances: no data
- Intervals of calibration: no data

REPETITIONS
- Number of determinations: duplicate
Type:
log Koc
Value:
ca. 2.1 dimensionless
Temp.:
25 °C
Remarks on result:
other: No data for organic carbon.
Type:
Koc
Value:
ca. 140 dimensionless
Temp.:
25 °C
Remarks on result:
other: No data for organic carbon
Details on results (HPLC method):
- Retention times of reference substances used for calibration: Acetanilide 3.556 min, Nitrobenzene 4.170 min, Naphthalene 5.154 min, 2-Methylnaphthalene 5.785 min, Anthracene 7.864 min, DDT 13.435 min.
- Average retention data for test substance: 4.442 min

A calibration plot of log10k versus log10Koc was constructed for the reference standards. The logarithm of the capacity factor for the test substance was then calculated and the log10Koc value was determined by interpolation.

Rikabinol HB was thus determined to have a log10Koc value of 2.1 (equivalent Koc value of 140).

Conclusions:
Rikabinol HB was found to have a log10Koc value of 2.1.

Description of key information

Rikabinol HB was found to have a log10Koc value of 2.1.

Key value for chemical safety assessment

Koc at 20 °C:
140

Additional information

The adsorption coefficient of HBPA was determined (Huntingdon Life Sciences, 2012, Study MOG0004) using high performance liquid chromatography (HPLC). The procedure was designed to be compatible the EC Method C.19 and the OECD test guidelines 121, and was conducted in compliance with GLP.

A solution of HBPA (approximately 0.5 g/l in HPLC mobile phase) was prepared and chromatographed in duplicate.

HBPA was determined to have a log10Koc value of 2.1 (equivalent Koc value of 140).