Isopulegol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Isopulegol

EC Number:

201-940-6

EC Name:

Isopulegol

Cas Number:

89-79-2

Molecular formula:

C10H18O

IUPAC Name:

isopulegol

Details on test material:

- Name of test substance: L-Isopulegol
- Test substance No.: 10/0358-2
- purity: 99.95 corr. area %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

- Age (on study day 0): A three day old preculture, prepared in dilution water, was used as inoculum.
- Supplier: Sammlung von Algenkulturen (SAG); Pflanzenphysiologisches Institut der Universität Göttingen; Nikolausberger Weg 18, D-37073 Göttingen

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Details on test conditions:

TEST SYSTEM
- Test vessel: Sterile headspace flasks, volume: 119 ml, aluminium tops with PTFE seals.
- Initial cells density: 7.085 x 10^3 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6


GROWTH MEDIUM
- Standard medium used: Nutrient medium Z according to LüTTGE et al. (1994)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 24h/d light
- Light intensity and quality: Approximately 60 - 120 μE · m-2 · s-1 (Fluorescent tubes, OSRAM L 36 W/11-865, cool daylight)

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

50.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

35.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes