Nitromethane

Specific investigations: other studies

Administrative data

Endpoint:

hepatotoxicity

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was not conducted according to guideline/s and GLP but the report contains sufficient data for interpretation of study results

Data source

Reference

Reference Type:

publication

Title:

Comparison of the Hepatotoxicity in Mice and the Mutagenicity of Three Nitroalkanes.

Author:

Dayal, R., Gescher, A., Harpur, E.S., Pratt, I., Chipman, J.K.

Year:

1989

Bibliographic source:

Fundamental and Applied Toxicology, 13:341-348.

Materials and methods

Principles of method if other than guideline:

Mice were injected with the test compounds via the IP route in a volume of 0.2 mL. Mice were sacrificed at 24, 48, or 96 hours post-dosing, and plasma was assayed for measurements of sorbitol dehydrogenase (SDH), alanine aminotransferase (ALT), and aspartate aminotransferase (AST). For histopathological investigation, livers were fixed, hydrated, and embedded. Sections from at least 3 lobes were cut and stained, and sections were evaluated blindly.

GLP compliance:

not specified

Type of method:

in vivo

Endpoint addressed:

not applicable

Test material

Test material information

Constituent 1

Reference substance name:

Nitromethane

EC Number:

200-876-6

EC Name:

Nitromethane

Cas Number:

75-52-5

Molecular formula:

CH3NO2

IUPAC Name:

nitromethane

Details on test material:

Purchased from Fluka Chemical Company (UK).

Test animals

Species:

mouse

Strain:

Balb/c

Sex:

male/female

Details on test animals or test system and environmental conditions:

Male or female BALB/c mice (19-25 g) were purchased from Bantin and Kingman Ltd. (UK) and fed on Heygate 41B breeding diet.

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

physiological saline

Details on exposure:

Mice were injected with the test material via the ip route in a volume of 0.2 ml. Nitromethane was injected at doses of 4.5, 6.7 or 9.0 mmol/kg; control mice were injected with NaCl (0.9% w/v).

Analytical verification of doses or concentrations:

not specified

Details on analytical verification of doses or concentrations:

No data.

Duration of treatment / exposure:

Single ip injection

Frequency of treatment:

Single ip injection

Post exposure period:

24, 48, 72 or 96 hours

Doses / concentrations

Remarks:

Doses / Concentrations:
4.5, 6.7, or 9.0 mmol/kg
Basis:
nominal conc.

No. of animals per sex per dose:

each group consisted of 3-5 mice.

Control animals:

yes, concurrent vehicle

Details on study design:

Mice were injected with the test compounds via the IP route in a volume of 0.2 mL. Mice were sacrificed at 24, 48, or 96 hours post-dosing, and plasma was assayed for measurements of sorbitol dehydrogenase (SDH), alanine aminotransferase (ALT), and aspartate aminotransferase (AST). For histopathological investigation, livers were fixed, hydrated, and embedded. Sections from at least 3 lobes were cut and stained, and sections were evaluated blindly.

Examinations

Examinations:

Mice were sacrificed at 24, 48, or 96 hours post-dosing, and plasma was assayed for measurements of sorbitol dehydrogenase (SDH), alanine aminotransferase (ALT), and aspartate aminotransferase (AST). For histopathological investigation, livers were fixed, hydrated, and embedded. Sections from at least 3 lobes were cut and stained, and sections were evaluated blindly.

Positive control:

No data

Results and discussion

Details on results:

The clinical chemistry measurements (SDH, ALT and AST activities) and histopathologic examination of livers of mice injected with NM did not show any significant abnormalities.

Any other information on results incl. tables

The clinical chemistry measurements (SDH, ALT and AST activities) and histopathologic examination of livers of mice injected with NM did not show any significant abnormalities.

Applicant's summary and conclusion

Conclusions:

The clinical chemistry measurements (SDH, ALT and AST activities) and histopathologic examination of livers of mice injected with NM did not show any significant abnormalities.

Executive summary:

The effects of nitromethane on liver clinical chemistry measurements and liver histopathology were examined in mice. The clinical chemistry measurements (SDH, ALT and AST activities) and histopathologic examination of livers of mice injected with NM did not show any significant abnormalities.