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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2009
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: test according to OECD301B under GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1,4 diaminobutane in water
- Physical state: clear colorless liquid
- Analytical purity: 67,91% w/w
- Composition of test material, percentage of components: 67,91% w/w 1,4 diaminobutane in water
- Lot/batch No.: DAB 81
- Stability under test conditions: stable
- Storage condition of test material: at room temperature in dark under nitrogen

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Fresh activated sludge from municipal sewage treatment plant: "Waterschap de Maaskant" at Den Bosch the Netherlands
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
32.5 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: mineral medium as described in OECD301B guideline
- Solubilising agent (type and concentration if used): Mili-RO water
- Test temperature: 21.3-24 °C
- pH: 7.2-8.1
- pH adjusted: yes
- Suspended solids concentration: 1% final volume of decanted inoculum (inoculum SS= 4.4 g/l, before use sludge allowed to settle for 80 min before decanting)
- Continuous darkness: no
- Other: IC < 5% of TC (mainly comning from test substance, 12 mg/l TOC)

TEST SYSTEM
- Number of culture flasks/concentration: 2 bottles test suspension, 2 bottles blank inoculum, 1 bottle positive control, 1 bottle toxicity control
- Method used to create aerobic conditions: sparging with synthetic air (CO2 < 1 ppm)
- Measuring equipment: titration with 0.05 M standarHCl and Phenolptalein as pH indicator
- Test performed in closed vessels due to significant volatility of test substance: yes
- Details of trap for CO2 and volatile organics if used: three CO2 absorbers with 100 ml 0.0125 M Ba(OH)2 in serie on the exit air line

SAMPLING
- Sampling frequency: every second or third day
- Sampling method: CO2-absorbers
- Sample storage before analysis: at room temperature in the dark under nitrogen

CONTROL AND BLANK SYSTEM
- Inoculum blank: only containing inoculum
- Toxicity control: test substance, reference substance and inoculum
- Positive control: reference substance and inoculum

STATISTICAL METHODS:
Reference substance
Reference substance:
acetic acid, sodium salt
Remarks:
purity > 99,0%, Supplier Merck, Art no 1.06268.0250

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
>= 89
Sampling time:
28 d

Any other information on results incl. tables

In the toxicity control more than 25% biodegradation occured within 14 days (71% based on ThCO2). Therefore, the test substance was assumed not to inhibit microbial activity. The positive control substance was biodegraded for 77% within 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
criteria of OECD301B and ISO standard 9439:
Interpretation of results:
readily biodegradable
Conclusions:
1,4 diaminobutane (67%) is designated as readily biodegradable
Executive summary:

The relative biodegradation values calculated from the measurements performed during the test period revealed 82% and 89% biodegradation of 1,4 diaminobutane (67%) for A and B, respectively. Furthermore, biodegradation of 1,4 diaminobutane (67%) of at least 60% was reached within a 10 -day window. In the toxicity control, 1,4 diaminobutane (67%) was found no to inhibit microbial activity. Since all criteria for acceptability of the test were met, this study is considered to be valid. In conclusion 1,4 diaminobutane (67%) is designated a readily biodegradable