Registration Dossier
Registration Dossier
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EC number: 942-293-6 | CAS number: -
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-03-01 - 1990-03-20
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test follows guideline, some deficiences in reporting
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Ethanol, 2,2'-iminobis-, N-coco alkyl derivs., N-oxides
- EC Number:
- 263-180-1
- EC Name:
- Ethanol, 2,2'-iminobis-, N-coco alkyl derivs., N-oxides
- Cas Number:
- 61791-47-7
- Molecular formula:
- not applicable
- IUPAC Name:
- N,N-Bis(2-hydroxyethyl)-C12-18(even numbered) alkyl-1-amine oxides
- Details on test material:
- Akzo No.; 1-036-013
Product code: 56508
Sample code: 12
Batch No.: 900110
Certificate of analysis: 1990-03-05
Amine oxide content: 30.5%
Free amine: 0.3%
Free peroxide: 0.13%
pH (10+90 water): 7.1
Colour: <2 (Gard.)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- n.a., measurement of extinction
Test solutions
- Vehicle:
- no
- Details on test solutions:
- no
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- Pseudomonas putida 50026
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 18 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- no data
- Test temperature:
- 25 deg C
- pH:
- Potassium hydrogenphosphate KH2PO4/Di Potassium hydrogenphosphate K2HPO4 buffer
- Dissolved oxygen:
- no data
- Salinity:
- no
- Nominal and measured concentrations:
- Nominal test substance (mg/L): 100-500-1000-3000-10000, Control (mg/L): 0
- Details on test conditions:
- according guideline
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 18 h
- Dose descriptor:
- EC10
- Effect conc.:
- 702 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Details on results:
- graphical evaluation
- Results with reference substance (positive control):
- n.a.
- Reported statistics and error estimates:
- n.a.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The registration substance has shown a low toxicity to Pseudomonas putida in an 18h growth inhibition test according DIN 38412, part 8 resulting in an EC10 (18h) of 2300 mg/L (nominal, test substance) or 702 mg/L (nominal, active ingredient).
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